Cyclometalated iridium(III) complexes containing an anthracene unit for sensing and imaging singlet oxygen in...

Singlet oxygen (¹O₂), as a highly reactive oxygen species, plays an important role in the physical, chemical and biomedical fields, especially during photodynamic therapy (PDT) process. In this work, two iridium(III) complexes containing an anthracene unit in their diimine ligand were designed and synthesized to monitor ¹O₂ in living cells. The complexes were weakly emissive owing to the photoinduced electron transfer process, but exhibited intense luminescence upon capturing ¹O₂, resulting from the formation of the corresponding endoperoxide analogues. The remarkable turn-on luminescence response was specific toward ¹O₂ and in preference to other reactive oxygen species. The utilization of one of the complexes for imaging ¹O₂ in living cells has also been demonstrated using three different cells lines. Cells incubated with the complexes were hardly emissive. Further light irradiation at 475 nm triggered intracellular emission turn on, indicative of the production of ¹O₂ photochemically. The emissive pattern was well colocalized with commercially available MitoTracker, suggesting the potential applications of the complexes for imaging mitochondria ¹O₂. The ¹O₂ capturing properties rendered the complexes low photocytotoxicity since ¹O₂-caused oxidative damage toward cellular molecules and structures was inhibited.