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Cyanine-based fluorescent indicator for mercury ion and bioimaging application in living cells
Guangjin Li1, Yihan Guan1, Fengying Ye1
1Key Laboratory of Pesticide and Chemical Biology, Ministry of Education, Hubei International Scientific and Technological Cooperation Base of Pesticide and Green Synthesis, International Joint Research Center for Intelligent Biosensing Technology and Health, College of Chemistry, Central China Normal University, Wuhan 430079, PR China.
Abstract:
A commercial cyanine dye IR-780 and a thioether-containing dicarboxylic acid ligand were used to construct the near-infrared fluorescent probe, which was used as a near-infrared fluorescent indicator for the determination of mercury ions in water and in living cells. This indicator displayed high specificity towards Hg2+ without any interference from other detecting species. Especially, the emission at 790 nm dramatically increased more than 25 times after interacting with Hg2+. The binding experiment showed that the indicator formed 1:1 complex with Hg2+. More, this indicator could be applied in the visualization of Hg2+ in living cells and measuring the Hg2+ concentration of tap-water sample.
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