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Correlation between TLR4 gene polymorphism and severe enterovirus 71 infection
Zhenzhen Chen1, Na Zhao2, Yulong Dai3
1Department of Pediatrics, Qingdao Women and Children's Hospital, Qingdao, 266000, China; Department of Pediatrics, The Affiliated Hospital of Qingdao University, Qingdao, 266000, China.
Insights
A specific Toll-like receptor 4 (TLR4) gene variation (rs10759932 C allele) is linked to severe Enterovirus 71 (EV71) infection outcomes in Chinese children, potentially increasing disease severity.
Area of Science:
- Immunology
- Genetics
- Pediatrics
Background:
- Enterovirus 71 (EV71) infection poses a significant threat to children, causing severe illness and death.
- Genetic factors, including immune response deficiencies and cytokine predispositions, are implicated in EV71 infection severity.
Purpose of the Study:
- To investigate the association between Toll-like receptor 4 (TLR4) gene polymorphisms and the clinical severity of EV71 infection in Chinese children.
Main Methods:
- TLR4 gene polymorphisms were identified using improved multiplex ligation detection reaction technology.
- TLR4 expression levels were quantified via Real-Time reverse transcriptase PCR and flow cytometry.
- Plasma concentrations of tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6) were measured using enzyme-linked immunosorbent assay.
Main Results:
- The rs10759932 CC genotype and C allele of TLR4 were more prevalent in children with severe EV71 infection compared to those with mild infection.
- Elevated levels of TLR4, serum TNF-α, and IL-6 were observed in patients with the rs10759932 CC genotype.
Conclusions:
- The rs10759932 polymorphism within the TLR4 gene is associated with EV71 infection severity.
- The C allele of rs10759932 may represent a genetic risk factor for severe EV71 infection in the pediatric population in China.
Objective:
Enterovirus 71 (EV71) infection resulted in high mortality and disability in children. Immune response deficiency and cytokine genetic predispositions were associated with the severity of EV71 infection. We aim to evaluate the association between TLR4 gene polymorphisms and severity of EV71 infection in Chinese children.
Methods:
TLR4 gene polymorphisms were detected through improved multiplex ligation detection reaction technology. TLR4 expression was measured by Real-Time reverse transcriptase PCR and flow cytometry. The plasma levels of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) were analyzed by enzyme-linked immunosorbent assay.
Results:
The frequency of rs10759932CC genotype and the C allele was significantly higher in cases with severe EV71 infection than those with mild infection. The levels of TLR4, serum TNF-α and IL-6 in cases with rs10759932CC genotype were also significantly elevated when compared to those with TT genotypes.
Conclusions:
The rs10759932 polymorphism in TLR4 was associated with the severity of EV71 infection. The C allele of rs10759932 may be one of the risk factors of severe EV71 infection in Chinese children.
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