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Related Experiment Video

Updated: Dec 20, 2025

Author Spotlight: Optimizing iPSC Differentiation for Efficient Production to Generate Kidney Organoids

Author Spotlight: Optimizing iPSC Differentiation for Efficient Production to Generate Kidney Organoids

Published on: September 1, 2023

Generating Kidney Organoids from Human Pluripotent Stem Cells Using Defined Conditions.

Sara E Howden^1,2, Melissa H Little^3,4

¹Murdoch Children's Research Institute, Parkville, VIC, Australia. sara.howden@mcri.edu.au.

Methods in Molecular Biology (Clifton, N.J.)

|June 1, 2020

Summary

Researchers developed a cost-effective protocol using human pluripotent stem cells (hPSCs) to generate kidney organoids. These organoids mimic human kidney tissue, advancing regenerative medicine for kidney disorders.

Keywords:

Directed differentiation Human pluripotent stem cell Kidney development Kidney organoid Nephrogenesis

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Last Updated: Dec 20, 2025

Author Spotlight: Optimizing iPSC Differentiation for Efficient Production to Generate Kidney Organoids

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A Simplified Method for Generating Kidney Organoids from Human Pluripotent Stem Cells

A Simplified Method for Generating Kidney Organoids from Human Pluripotent Stem Cells

Published on: April 13, 2021

Generation of Human Kidney Tubuloids from Tissue and Urine

Generation of Human Kidney Tubuloids from Tissue and Urine

Published on: April 16, 2021

Area of Science:

Regenerative Medicine
Stem Cell Biology
Developmental Biology
Nephrology

Background:

Regenerative medicine aims to generate specific cell types for treating intractable disorders.
Human pluripotent stem cells (hPSCs) offer potential for creating therapeutic cell types.
Previous work established protocols for generating kidney organoids from hPSCs.

Purpose of the Study:

To describe an updated, cost-effective, and defined xeno-free differentiation protocol for kidney organoids.
To generate complex, multicellular three-dimensional kidney tissue structures from hPSCs.

Main Methods:

Utilized human pluripotent stem cells (hPSCs).
Employed a directed differentiation protocol using a cost-effective, defined, xeno-free medium.
Characterized the resulting three-dimensional kidney organoids.

Main Results:

Successfully generated kidney organoids composed of all anticipated kidney cell types.
Organoids include nephron segments (glomerulus, proximal and distal tubules), an endothelial network, and renal interstitium.
The protocol uses a defined, xeno-free medium, enhancing cost-effectiveness and safety.

Conclusions:

Kidney organoids derived from hPSCs are valuable tools for studying human kidney development and disease.
These organoids are applicable for drug screening, toxicology studies, and tissue engineering.
This protocol facilitates the development of transplantable hPSC-derived kidney tissue for regenerative medicine.