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FlICk (fluorescent isoindole crosslinking) for peptide stapling.

Mihajlo Todorovic¹, David M Perrin¹

¹Department of Chemistry, University of British Columbia, Vancouver, BC, Canada.

Methods in Enzymology

|June 2, 2020

Summary

This summary is machine-generated.

This study introduces FlICk (fluorescent isoindole crosslink) chemistry, a user-friendly method for rigidifying peptides. This technique creates fluorescent or colored peptide staples for biochemical and pharmaceutical applications, enhancing cellular function probes.

Keywords:

Fluorescence Isoindole Macrocycle Ortho-phthalaldehyde Peptide stapling

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Area of Science:

Medicinal Chemistry
Biochemistry
Organic Synthesis

Background:

Peptide structure modification is crucial for developing functional peptides in medicine and biochemistry.
Incorporating fluorophores and chromophores into peptides is valuable for creating probes to study cellular function and localization.
Peptide stapling rigidifies structure, enhancing stability and biological activity.

Purpose of the Study:

To introduce and detail a novel method, FlICk (fluorescent isoindole crosslink) chemistry, for creating fluorescent peptide staples.
To demonstrate the versatility of FlICk chemistry in modifying peptide structures for various applications.
To showcase the synthesis of biologically active peptide mimics using this approach.

Main Methods:

Utilizing ortho-phthalaldehydes to form isoindole staples, crosslinking peptide precursors.
Applying FlICk chemistry to high-affinity macrocyclic α-MSH derivatives.
Performing late-stage intra-annular isoindole stapling to create a bicyclic peptide mimic of α-amanitin.

Main Results:

Transformed inactive linear and monocyclic peptide precursors into fluorescent or colored monocyclic and bicyclic products.
Developed a user-friendly method (FlICk chemistry) for peptide rigidification and fluorescent labeling.
Synthesized a bicyclic peptide mimic of α-amanitin with demonstrated cytotoxicity to CHO cells.

Conclusions:

FlICk chemistry offers a versatile and user-friendly approach for peptide rigidification and fluorescent labeling.
This method enables the creation of novel peptide-based probes and therapeutics with enhanced biological activity.
The synthetic strategies for ortho-phthalaldehydes and their application in FlICk stapling are comprehensively detailed.