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Updated: Dec 20, 2025

Assessment of Global DNA Double-Strand End Resection using BrdU-DNA Labeling coupled with Cell Cycle Discrimination Imaging
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Targeting Mouse Double Minute 2: Current Concepts in DNA Damage Repair and Therapeutic Approaches in Cancer

Wen Li1, Xinhao Peng1,2, Jinyi Lang1,2

  • 1Cancer Clinical Research Center & Integrative Cancer Center, Sichuan Cancer Hospital & Institute Sichuan Cancer Center, School of Medicine, University of Electronic Science and Technology of China, Chengdu, China.

Frontiers in Pharmacology
|June 2, 2020
PubMed

Insights

Defects in DNA repair can lead to cancer. This review details how mouse double minute 2 (MDM2) impacts genomic instability by inhibiting p53 and DNA repair proteins, affecting cancer progression and treatment.

Area of Science:

  • Molecular Biology
  • Genetics
  • Oncology

Background:

  • DNA damage repair defects are linked to genome instability and cancer.
  • The tumor suppressor p53 is crucial for cell cycle arrest and DNA repair.
  • The oncoprotein MDM2 inhibits p53 and promotes cancer cell survival and proliferation.

Purpose of the Study:

  • To review the influence of MDM2 on genomic instability.
  • To elucidate MDM2's role in inhibiting p53 and DNA repair.
  • To discuss MDM2's regulatory network and oncogenic functions.

Main Methods:

  • Literature review of MDM2's function in DNA damage response.
  • Analysis of MDM2's interactions with p53 and DNA repair proteins.
  • Examination of MDM2's regulatory network and oncogenic pathways.

Main Results:

  • MDM2 significantly contributes to genomic instability by suppressing p53 and DNA repair.
  • MDM2 regulates its own stability, localization, and transcriptional modifications post-DNA damage.
  • MDM2 exhibits both p53-dependent and independent oncogenic functions.

Conclusions:

  • MDM2 plays a critical role in maintaining genome integrity and cancer development.
  • Targeting the MDM2-p53 axis offers a promising therapeutic strategy for various cancers.
  • Clinical trials investigating MDM2 inhibitors show potential for cancer treatment.
Keywords:
DNA damage repairMDM2P53clinical inhibitorgenomic instability

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