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Highly Efficient Cell Membrane Tracker Based on a Solvatochromic Dye with Near-Infrared Emission
Huijie Liu1, Hongyi Zhang1, Jiajie Luo1
1Department of Pharmacology and Medicinal Chemistry, Qingdao University School of Pharmacy, Qingdao 266021, China.
ACS Omega
|June 2, 2020
Summary
Researchers developed a novel fluorescent probe, MRL, to specifically label and monitor cell membrane morphology changes over time. This tool aids in identifying unhealthy cells by detecting abnormal plasma membrane shapes.
Area of Science:
- Cell Biology
- Biochemistry
- Fluorescence Imaging
Background:
- Cell membranes are crucial for homeostasis, and abnormal shapes indicate cellular dysfunction.
- Current imaging probes lack the specificity and long-term monitoring capabilities for dynamic cell membrane changes.
Purpose of the Study:
- To design and synthesize a novel amphipathic fluorescent probe for specific cell membrane staining.
- To evaluate the probe's efficacy in monitoring dynamic changes in cell membrane morphology.
Main Methods:
- Synthesis of an amphipathic molecule (MRL) based on a solvatochromic dye with near-infrared emission.
- Incubation of cells with MRL for specific cell membrane labeling.
- Long-term monitoring of cell membrane morphology using fluorescence imaging.
Main Results:
- MRL specifically stained cell membranes with excellent photostability and low cytotoxicity.
- The probe enabled long-term monitoring of morphological changes in cell membranes under various conditions.
- Abnormal cell membrane shapes were identifiable using MRL-based imaging.
Conclusions:
- MRL is a promising fluorescent probe for specifically labeling cell membranes.
- MRL facilitates the long-term observation of dynamic cell membrane morphology, aiding in the identification of unhealthy cells.
