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Svetlana Kucher1, Sergej Korneev2, Johann P Klare1

  • 1Department of Physics, Osnabrück University, Barbarastr. 7, Osnabrück, Germany. jklare@uni-osnabrueck.de hsteinho@uni-osnabrueck.de.

Physical Chemistry Chemical Physics : PCCP
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Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...