Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

12.1K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
12.1K
Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

8.6K
Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
8.6K
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

19.7K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
19.7K
Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

684
Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
684

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A light-initiated chemical reporter strategy for spatiotemporal labeling of biomolecules.

RSC chemical biology·2022
Same author

CTBP1‑AS upregulation is associated with polycystic ovary syndrome and can be effectively downregulated by cryptotanshinone.

Molecular medicine reports·2022
Same author

Downregulation of HULC Induces Ferroptosis in Hepatocellular Carcinoma via Targeting of the miR-3200-5p/ATF4 Axis.

Oxidative medicine and cellular longevity·2022
Same author

Effect of Programmed Nursing Plan Based on Thinking Map Guidance Mode on Hemodynamics and Intestinal Function Recovery of Patients Undergoing Endoscopic Retrograde Cholangiopancreatography.

Emergency medicine international·2022
Same author

In vivo non-invasive confocal fluorescence imaging beyond 1,700 nm using superconducting nanowire single-photon detectors.

Nature nanotechnology·2022
Same author

Primary signet ring cell carcinoma of the appendix: An interesting case.

The American journal of the medical sciences·2022

Related Experiment Video

Updated: Dec 20, 2025

Demonstration of a Hyperlens-integrated Microscope and Super-resolution Imaging
10:01

Demonstration of a Hyperlens-integrated Microscope and Super-resolution Imaging

Published on: September 8, 2017

8.1K

Enhanced high-quality super-resolution imaging in air using microsphere lens groups.

Hao Luo, Haibo Yu, Yangdong Wen

    Optics Letters
    |June 2, 2020
    PubMed
    Summary

    This study introduces a novel microsphere lens group (MLG) for super-resolution imaging in air, avoiding liquid contamination. This air-based method achieves high-quality imaging comparable or superior to traditional liquid-immersed techniques.

    More Related Videos

    Super-Resolution Live Cell Imaging of Subcellular Structures
    06:50

    Super-Resolution Live Cell Imaging of Subcellular Structures

    Published on: January 13, 2021

    5.2K
    Confocal and Super-Resolution Imaging of Polarized Intracellular Trafficking and Secretion of Basement Membrane Proteins During Drosophila Oogenesis
    10:41

    Confocal and Super-Resolution Imaging of Polarized Intracellular Trafficking and Secretion of Basement Membrane Proteins During Drosophila Oogenesis

    Published on: May 19, 2022

    2.5K

    Related Experiment Videos

    Last Updated: Dec 20, 2025

    Demonstration of a Hyperlens-integrated Microscope and Super-resolution Imaging
    10:01

    Demonstration of a Hyperlens-integrated Microscope and Super-resolution Imaging

    Published on: September 8, 2017

    8.1K
    Super-Resolution Live Cell Imaging of Subcellular Structures
    06:50

    Super-Resolution Live Cell Imaging of Subcellular Structures

    Published on: January 13, 2021

    5.2K
    Confocal and Super-Resolution Imaging of Polarized Intracellular Trafficking and Secretion of Basement Membrane Proteins During Drosophila Oogenesis
    10:41

    Confocal and Super-Resolution Imaging of Polarized Intracellular Trafficking and Secretion of Basement Membrane Proteins During Drosophila Oogenesis

    Published on: May 19, 2022

    2.5K

    Area of Science:

    • Optics and Photonics
    • Super-resolution Microscopy
    • Nanotechnology

    Background:

    • Microsphere-assisted super-resolution imaging typically requires high-refractive-index microspheres immersed in liquid.
    • Liquid immersion can lead to sample contamination from residuals.
    • Existing methods face limitations in environments where liquids are impractical.

    Purpose of the Study:

    • To present a novel method for achieving high-quality super-resolution imaging in air using a microsphere lens group (MLG).
    • To demonstrate that the MLG method offers performance comparable or superior to liquid-immersed microsphere techniques.
    • To provide an alternative for microsphere imaging applications where liquid use is not feasible.

    Main Methods:

    • Development and implementation of a microsphere lens group (MLG) for optical imaging.
    • Utilizing the MLG to achieve super-resolution imaging directly in an air environment.
    • Characterization of the imaging performance and comparison with liquid-immersed microsphere methods.

    Main Results:

    • The MLG enables high-quality super-resolution imaging in air without liquid immersion.
    • Performance of the MLG method is on par with or exceeds that of liquid-immersed high-refractive-index microspheres.
    • The MLG generates a real image linked to the photonic nanojet of the microsphere super-lens.

    Conclusions:

    • The MLG offers a practical and effective solution for super-resolution imaging in air.
    • This novel approach overcomes the limitations of liquid-based microsphere imaging, preventing sample contamination.
    • The MLG is a valuable tool for applications requiring microsphere imaging where liquids are impractical.