[Evaluation of cascade filtration on a test sample]

Double filtration or filtration in series can be consider as an effective means of removing proteins such as IgG, IgM, immune complexes, lipoproteins from plasma. In this study, we evaluated, using a biological and technical protocol: Kuraray (2A and 4A) and Dideco (Albusave) filters. Results were good by analysis of the sieving coefficient, but the method had 2 inconveniences: problem of slogging of the columns after filtering 2 liters and too high a reject coefficient of albumin. No effective solutions exist to avoid these two problems. Nevertheless, the filtration in series technic seems to be a good method for removing cholesterol and we have undertaken new studies to improve this system.