Open-source raspberry Pi-based operant box for translational behavioral testing in rodents.

Area of Science:

• Neuroscience
• Behavioral Science
• Biomedical Engineering

Background:

• Rodents are crucial models for studying neural circuits underlying behavior.
• Standardized training paradigms and human-comparable assays are increasingly important.
• Existing commercial systems are costly and not optimized for electrophysiology.

Purpose of the Study:

• To develop an inexpensive, customizable operant chamber for rodent behavioral research.
• To integrate electrophysiological and optogenetic capabilities into an accessible system.
• To provide open-source resources for building and operating the chambers.

Main Methods:

• Utilized Raspberry Pi for an affordable (<$1000) visual-screen operant chamber.
• Integrated electrophysiological and optogenetic compatibility.
• Operated the chamber using MATLAB/Simulink for customization.

Main Results:

• Demonstrated successful data collection for behavior and electrophysiology.
• Provided comprehensive resources including CAD models, PCB designs, and bill of materials.
• Showcased the system's ability to combine automated behavioral testing with high-temporal-precision electrophysiological readouts.

Conclusions:

• The developed open-source operant chambers are significantly more cost-effective than commercial alternatives.
• The system facilitates high-throughput visual/auditory behavioral assays at approximately 1/10th the cost.
• Enables advanced neuroscience research by combining behavioral testing with electrophysiology.