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Related Concept Videos

Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

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Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
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Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

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Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
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MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

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Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
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Mass Spectrometry: Overview01:19

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Mass spectrometry is an analytical technique used to determine the molecular mass and molecular formula of a compound. The basic principle of mass spectrometry is to generate ions from the analyte molecule and measure these ion abundances against their molecular mass. One common type of ionization, known as electron ionization or EI, bombards the analyte molecules in the gas phase with high-energy electron beams. The electron beams displace an electron from the molecule and leave behind a...
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Mass Spectrometers01:16

Mass Spectrometers

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This lesson details the instrumentation of a mass spectrometer—a physical instrument to perform mass spectrometry on analyte molecules and record the characteristic mass spectra. This is achieved via three chief functions:
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Mass Analyzers: Overview01:13

Mass Analyzers: Overview

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The mass analyzer is a crucial component of the mass spectrometer. In the ionization chamber, the vaporized sample is bombarded with a high-energy electron beam to generate a radical cation and further fragment into neutral molecules, radicals, and cations. A series of negatively charged accelerator plates accelerate the cations into the mass analyzer. The mass analyzer separates ions according to their mass-to-charge (m/z) ratios and then directs them to the detector. The common types of mass...
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Related Experiment Video

Updated: Dec 20, 2025

Combining Chemical Cross-linking and Mass Spectrometry of Intact Protein Complexes to Study the Architecture of Multi-subunit Protein Assemblies
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Harmonizing structural mass spectrometry analyses in the mass spec studio.

Daniel S Ziemianowicz1, Vladimir Sarpe2, D Alex Crowder1

  • 1Department of Biochemistry and Molecular Biology, University of Calgary, Calgary, Alberta T2N 4N1, Canada; Robson DNA Science Centre, Arnie Charbonneau Cancer Institute, University of Calgary, Calgary, Alberta T2N 4N1, Canada.

Journal of Proteomics
|June 2, 2020
PubMed
Summary
This summary is machine-generated.

Structural Mass Spectrometry (SMS) offers tools for protein analysis. The Mass Spec Studio enhances SMS workflows for integrative modeling, providing crucial computational resources for researchers.

Keywords:
Covalent labelingCrosslinkingHydrogen/deuterium exchangeIntegrative modelingStructural mass spectrometry

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Area of Science:

  • Biochemistry
  • Structural Biology
  • Computational Biology

Background:

  • Structural Mass Spectrometry (SMS) is vital for protein structure and function analysis.
  • Integrative structural modeling of complex protein systems benefits from SMS data.
  • Existing computational tools for SMS data interpretation are insufficient, especially for large systems.

Purpose of the Study:

  • To review new developments in the Mass Spec Studio for SMS data analysis.
  • To highlight the Studio's role in supporting integrative structural modeling.
  • To address the need for improved computational resources in the SMS field.

Main Methods:

  • Review of new functionalities within the Mass Spec Studio (version 2).
  • Focus on workflows for crosslinking, hydrogen/deuterium exchange, and covalent labeling.
  • Examination of pipelines for structural modeling and data integration.

Main Results:

  • The Mass Spec Studio (version 2) now supports three major SMS workflows and two structural modeling pipelines.
  • New capabilities include enhanced analysis of crosslinking, hydrogen/deuterium exchange, and covalent labeling.
  • A utility for translating SMS analyses into restraints for integrative structural modeling has been developed.

Conclusions:

  • The Mass Spec Studio provides an expandable ecosystem for SMS data analysis and modeling.
  • Updates enhance the analysis of key SMS techniques and their integration into modeling.
  • The Studio offers valuable resources for tackling complex structural problems and advancing data analysis approaches in structural proteomics.