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Analysis of Cell Cycle Position in Mammalian Cells
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Multiple Functions of Fubp1 in Cell Cycle Progression and Cell Survival

Mingyu Kang1, Hyeon Ji Kim1, Tae-Jun Kim1

  • 1Department of Pharmacology, School of Dentistry, Kyungpook National University, 700-412 Daegu, Korea.

Cells
|June 3, 2020
PubMed

Insights

The study reveals that Fubp1 protein levels are high during S phase, impacting cell cycle progression. Fubp1 deficiency offers cancer cells survival advantages against stress and drugs, highlighting its dual role in cancer.

Area of Science:

  • Molecular biology
  • Cancer research
  • Cell cycle regulation

Background:

  • Double-agent genes possess both oncogenic and tumor-suppressive functions, with fewer than 100 documented.
  • Fubp1 acts as a master transcriptional regulator by interacting with the FUSE element.
  • Fubp1's dual roles in tumorigenesis are debated, necessitating detailed mechanistic studies.

Purpose of the Study:

  • To investigate the molecular mechanisms of Fubp1 in cancer development.
  • To determine the role of Fubp1 in cell cycle progression and its association with cyclin A.
  • To explore Fubp1's impact on cancer cell survival under stress and drug treatment.

Main Methods:

  • Analysis of Fubp1 protein levels during the cell cycle.
  • Assessment of Fubp1's effect on cell cycle progression, particularly S phase.
  • Quantification of Ccna gene (cyclin A) mRNA expression in Fubp1-deficient cells.
  • Evaluation of Fubp1's influence on cancer cell survival against metabolic stress and anti-cancer drugs.

Main Results:

  • Fubp1 protein levels are enriched in the S phase of the cell cycle.
  • Fubp1 deficiency alters cell cycle progression, specifically downregulating cyclin A (Ccna) mRNA expression.
  • Fubp1 exhibits heterogeneous expression across various cancer tissues, indicating complex functions.
  • Fubp1 deficiency enhances cancer cell survival against metabolic stress and anti-cancer drugs.

Conclusions:

  • Fubp1 plays a critical role in regulating cell cycle progression, particularly during S phase, via the cyclin A pathway.
  • Fubp1's expression pattern and functions are context-dependent and heterogeneous across different cancers.
  • Fubp1 deficiency confers a survival advantage to cancer cells, suggesting a complex, potentially dual role in malignant development.
Keywords:
Fubp1cell cyclecell deathcyclin Adouble-agentlung cancer

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