Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Stem Cell Culture01:17

Stem Cell Culture

5.9K
Stem cell research aims to find ways to use stem cells to regenerate and repair cellular damage. Over time, most adult cells undergo the wear and tear of aging and lose their ability to divide and repair themselves. Stem cells do not display a particular morphology or function. Adult stem cells, which exist as a small subset of cells in most tissues, keep dividing and can differentiate into a number of specialized cells generally formed by that tissue. These cells enable the body to renew and...
5.9K
Cell Culture01:21

Cell Culture

21.6K
Most vertebrate cells grow in vitro attached to a substrate as a monolayer, called adherent cultures. The flasks and plates used to grow cells are chemically treated to facilitate cell attachment. However, a few cell types, such as hematopoietic cells, can grow in a suspension. In contrast to adherent cultures, suspension cultures can grow in non-treated cultureware using magnetic stirrers or spinner flasks to agitate the culture media
21.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

High-Resolution Molecular Analyses Reveal Non-additive Impacts of Chronic Warming and Nitrogen Addition on Soil-Derived Dissolved Organic Matter.

Environmental science & technology·2026
Same author

Edge computing-based computer vision and deep transfer learning for high-throughput assessment of <i>Aspergillus flavus</i> infection in crop seeds.

Plant phenomics (Washington, D.C.)·2026
Same author

Corrigendum to 'Composite scaffolds of black phosphorus nanosheets and gelatin with controlled pore structures for photothermal cancer therapy and adipose tissue engineering' [Biomaterials 275 (2021) 120923].

Biomaterials·2026
Same author

Peptide modified lipid nanoparticles Co-Delivering IL-12 mRNA and PD-L1 siRNA for glioblastoma immunotherapy.

International journal of pharmaceutics·2026
Same author

Influence of microenvironmental viscosity on the cellular uptake of Fe<sub>3</sub>O<sub>4</sub> nanoparticles and their anticancer effect.

Nanoscale·2026
Same author

Preterm Birth International Collaborative Australasia Branch: Expert Consensus on Diagnosis and Treatment of Neonatal Lactose Intolerance (2025).

Pediatric discovery·2026
Same journal

Fiber and continuum scale contributions to the intrinsic and apparent fracture of soft collagenous tissue <i>via</i> cutting.

Biomaterials science·2026
Same journal

Surface morphology-regulated tissue adhesion in solid and mesoporous silica-reinforced gelatin nanocomposite hydrogels.

Biomaterials science·2026
Same journal

Nanostructured hyaluronic acid-chia mucilage film as bioactive wound dressings for accelerated skin regeneration.

Biomaterials science·2026
Same journal

Tunable bio-inspired hybrid hydrogels reprogram stem cell-derived extracellular vesicles for superior wound regeneration.

Biomaterials science·2026
Same journal

Bioorthogonally reinforced injectable granular hydrogels synergizing ECM mimicry with microporosity for skin tissue engineering.

Biomaterials science·2026
Same journal

Modeling a hypoxia-integrated glioblastoma microenvironment to mimic tumor heterogeneity and chemoresistance.

Biomaterials science·2026
See all related articles

Related Experiment Video

Updated: Dec 20, 2025

Alternative Cultures for Human Pluripotent Stem Cell Production, Maintenance, and Genetic Analysis
08:27

Alternative Cultures for Human Pluripotent Stem Cell Production, Maintenance, and Genetic Analysis

Published on: July 24, 2014

12.8K

Stem cell culture using cell-derived substrates.

Binata Joddar1, Takashi Hoshiba, Guoping Chen

  • 1Nano Medical Engineering Laboratory, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan. y-ito@riken.jp.

Biomaterials Science
|June 3, 2020
PubMed
Summary
This summary is machine-generated.

New cell culture substrates derived from cells and extracellular matrix (ECM) show promise for regulating stem cell functions. These biomaterials offer advanced approaches for stem cell culture and regenerative medicine applications.

More Related Videos

Efficient Neural Differentiation using Single-Cell Culture of Human Embryonic Stem Cells
11:17

Efficient Neural Differentiation using Single-Cell Culture of Human Embryonic Stem Cells

Published on: January 18, 2020

10.7K
Human Pluripotent Stem Cell Culture on Polyvinyl Alcohol-Co-Itaconic Acid Hydrogels with Varying Stiffness Under Xeno-Free Conditions
11:37

Human Pluripotent Stem Cell Culture on Polyvinyl Alcohol-Co-Itaconic Acid Hydrogels with Varying Stiffness Under Xeno-Free Conditions

Published on: February 3, 2018

9.6K

Related Experiment Videos

Last Updated: Dec 20, 2025

Alternative Cultures for Human Pluripotent Stem Cell Production, Maintenance, and Genetic Analysis
08:27

Alternative Cultures for Human Pluripotent Stem Cell Production, Maintenance, and Genetic Analysis

Published on: July 24, 2014

12.8K
Efficient Neural Differentiation using Single-Cell Culture of Human Embryonic Stem Cells
11:17

Efficient Neural Differentiation using Single-Cell Culture of Human Embryonic Stem Cells

Published on: January 18, 2020

10.7K
Human Pluripotent Stem Cell Culture on Polyvinyl Alcohol-Co-Itaconic Acid Hydrogels with Varying Stiffness Under Xeno-Free Conditions
11:37

Human Pluripotent Stem Cell Culture on Polyvinyl Alcohol-Co-Itaconic Acid Hydrogels with Varying Stiffness Under Xeno-Free Conditions

Published on: February 3, 2018

9.6K

Area of Science:

  • Biomaterials Science
  • Stem Cell Biology
  • Tissue Engineering

Background:

  • Cell culture substrates are crucial for regulating stem cell functions like proliferation and differentiation.
  • In vivo, stem cells interact with dynamic microenvironments comprising cells and extracellular matrix (ECM).
  • Mimicking these microenvironments in vitro is key for effective stem cell culture.

Purpose of the Study:

  • To review recent advancements in cell- and cell-formed ECM-derived substrates.
  • To highlight their potential as functional biomaterials for stem cell applications.
  • To discuss progress in mimicking in vivo stem cell microenvironments.

Main Methods:

  • Review of literature on cell- and ECM-derived substrates.
  • Analysis of substrate properties influencing stem cell behavior.
  • Comparison with traditional protein-coated substrates.

Main Results:

  • Cell- and cell-formed ECM-derived substrates demonstrate significant progress.
  • These substrates show great potential for stem cell culture and regenerative medicine.
  • They offer improved mimicry of native stem cell microenvironments compared to protein coatings.

Conclusions:

  • Cell- and ECM-derived substrates represent a promising frontier in stem cell research.
  • These biomaterials are advancing stem cell culture and regenerative medicine.
  • Further development holds potential for enhanced control over stem cell fate and function.