Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Notch Signaling Pathway03:14

Notch Signaling Pathway

6.2K
The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not...
6.2K
Cleavage and Blastulation01:33

Cleavage and Blastulation

49.4K
After a large-single-celled zygote is produced via fertilization, the process of cleavage occurs while zygotes travel through the uterine tube. Cleavage is a mitotic cell division that does not result in growth. With each round of successive cell division, daughter cells get increasingly smaller.
49.4K
Role Of Notch Signalling In Intestinal Stem Cell Renewal01:12

Role Of Notch Signalling In Intestinal Stem Cell Renewal

2.3K
Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
2.3K
Determination01:51

Determination

20.5K
During embryogenesis, cells become progressively committed to different fates through a two-step process: specification followed by determination. Specification is demonstrated by removing a segment of an early embryo, “neutrally” culturing the tissue in vitro—for example, in a petri dish with simple medium—and then observing the derivatives. If the cultured region gives rise to cell types that it would normally generate in the embryo, this means that it is specified. In...
20.5K
Hedgehog Signaling Pathway02:33

Hedgehog Signaling Pathway

9.5K
The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...
9.5K
Gastrulation01:56

Gastrulation

64.9K
Gastrulation establishes the three primary tissues of an embryo: the ectoderm, mesoderm, and endoderm. This developmental process relies on a series of intricate cellular movements, which in humans transforms a flat, “bilaminar disc” composed of two cell sheets into a three-tiered structure. In the resulting embryo, the endoderm serves as the bottom layer, and stacked directly above it is the intermediate mesoderm, and then the uppermost ectoderm. Respectively, these tissue strata...
64.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Spatial mapping of Notch signaling components along the bull genital tract and in epididymal and vas deferens vesicles.

Animal reproduction science·2026
Same author

Endometritis and endometrial fibrosis are associated with alterations in mare endometrial proteome.

BMC veterinary research·2026
Same author

Biomechanical characterization of the female rectum in vivo through MRI-based inverse finite element analysis.

Journal of biomechanics·2026
Same author

Real-world resource utilization, toxicity management, and efficacy outcomes of CD20 × CD3 bispecific antibodies in B-cell lymphoma: A study from the GELTAMO network.

HemaSphere·2026
Same author

Exploring the role of epigenetics in the processes related to the development of endometrosis in the mare.

The Journal of reproduction and development·2026
Same author

Effects of Defatted Black Soldier Fly (<i>Hermetia illucens</i>) Meal on the Performance, Digestibility and Blood Parameters of Weaned Piglets.

Animals : an open access journal from MDPI·2026

Related Experiment Video

Updated: Dec 20, 2025

Protocol for Human Blastoids Modeling Blastocyst Development and Implantation
12:09

Protocol for Human Blastoids Modeling Blastocyst Development and Implantation

Published on: August 10, 2022

7.1K

Notch signaling in mouse blastocyst development and hatching.

Mariana R Batista1, Patrícia Diniz1, Ana Torres1

  • 1Reproduction and Development Laboratory, CIISA - Centro de Investigação Interdisciplinar em Sanidade Animal, Faculdade de Medicina Veterinária, Universidade de Lisboa, 1300-477, Lisbon, Portugal.

BMC Developmental Biology
|June 3, 2020
PubMed
Summary

Notch signaling plays a dynamic role in mouse blastocyst development and hatching. Deregulation of this pathway, either over or under-activation, negatively impacts embryo development and hatching success.

Keywords:
BlastocystDevelopmentHatchingMouseNotch

More Related Videos

Blastomere Explants to Test for Cell Fate Commitment During Embryonic Development
14:08

Blastomere Explants to Test for Cell Fate Commitment During Embryonic Development

Published on: January 26, 2013

15.7K
Quantitative Analysis of Protein Expression to Study Lineage Specification in Mouse Preimplantation Embryos
11:25

Quantitative Analysis of Protein Expression to Study Lineage Specification in Mouse Preimplantation Embryos

Published on: February 22, 2016

11.2K

Related Experiment Videos

Last Updated: Dec 20, 2025

Protocol for Human Blastoids Modeling Blastocyst Development and Implantation
12:09

Protocol for Human Blastoids Modeling Blastocyst Development and Implantation

Published on: August 10, 2022

7.1K
Blastomere Explants to Test for Cell Fate Commitment During Embryonic Development
14:08

Blastomere Explants to Test for Cell Fate Commitment During Embryonic Development

Published on: January 26, 2013

15.7K
Quantitative Analysis of Protein Expression to Study Lineage Specification in Mouse Preimplantation Embryos
11:25

Quantitative Analysis of Protein Expression to Study Lineage Specification in Mouse Preimplantation Embryos

Published on: February 22, 2016

11.2K

Area of Science:

  • Developmental Biology
  • Cell Signaling
  • Genetics

Background:

  • Mammalian early embryo development relies on intricate cell signaling pathways.
  • The Notch pathway is crucial for cell-fate determination but its role in pre-implantation development is debated.
  • The precise function of Notch in blastocyst development and hatching, along with its temporal gene and protein expression, remains unclear.

Purpose of the Study:

  • To comprehensively analyze the dynamic transcription and protein expression of Notch components during mouse blastocyst development and hatching.
  • To investigate the relationship between Notch signaling components and key pluripotency/differentiation genes.
  • To elucidate the impact of manipulating Notch signaling on blastocyst development and hatching.

Main Methods:

  • Simultaneous analysis of individual embryo gene transcription and protein expression for Notch receptors, ligands, and effectors.
  • Correlation analysis with transcription of pluripotency (Sox2, Oct4, Klf4) and differentiation (Cdx2) markers.
  • Detection of Notch signaling activity via nuclear NICD and Hes1, and assessment of canonical signaling blockade using DAPT.
  • In vitro embryo culture with Jagged1/Jagged2 supplementation and Notch signaling blockade.

Main Results:

  • Notch1-2, Jagged1-2, and Hes1 showed dynamic and prevalent transcription, correlating with pluripotency and differentiation genes.
  • Protein expression generally mirrored transcription, except for Jagged2.
  • Notch signaling activity was confirmed, and Hes1 transcription decreased upon DAPT treatment.
  • Jagged1 supplementation had no effect, while Jagged2 inhibited hatching and downregulated Cdx2 transcription.
  • Notch blockade by DAPT reduced Sox2 transcription and delayed hatching.

Conclusions:

  • Notch gene transcription is dynamic during blastocyst development and hatching.
  • Notch canonical signaling likely involves Notch1, Notch3, Jagged1, and Hes1.
  • Jagged1 and Jagged2 influence Cdx2 transcription and blastocyst hatching.
  • Aberrant Notch signaling, either excessive or insufficient, impairs blastocyst development and hatching.