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Related Concept Videos

Golgi Apparatus01:49

Golgi Apparatus

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As they leave the Endoplasmic Reticulum (ER), properly folded and assembled proteins are selectively packaged into vesicles. These vesicles are transported by microtubule-based motor proteins and fuse together to form vesicular tubular clusters, subsequently arriving at the Golgi apparatus, a eukaryotic endomembrane organelle that often has a distinctive ribbon-like appearance.
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Golgi Apparatus01:09

Golgi Apparatus

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Properly folded and assembled proteins are selectively packaged into vesicles that exit the ER. Motor proteins transport these vesicles to the Golgi apparatus for adding modifications that make these proteins functional at their destination.
The Golgi apparatus is a eukaryotic organelle that has a distinctive ribbon-like appearance. It is a primary sorting and dispatch station for cargo arriving from the ER. Newly arriving vesicles enter the cis face of the Golgi, closest to the ER, and are...
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Golgi Matrix Proteins01:12

Golgi Matrix Proteins

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Golgi matrix proteins are a group of highly dynamic proteins that maintain the stacked structure of Golgi. These proteins adapt to rapid morphological changes of the Golgi during the cell cycle. During cell division, mild proteolysis removes these connections resulting in Golgi unstacking. In The daughter cells, these proteins help reassemble the unstacked Golgi.
One of the first identified Golgi matrix proteins was GM130, a rod-like protein located in the cis-Golgi. Subsequently, many Golgi...
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Transport Across the Golgi01:26

Transport Across the Golgi

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While it is unclear how molecules move between adjacent Golgi cisternae, it is apparent that the molecules move from cis- cisterna, the entry face, to the trans- cisterna, the exit face. Experiments initially suggested vesicles that bud from one cisterna and fuse with the next cisterna to transport proteins between the cisternae. This vesicular transport model describes the Golgi apparatus as a relatively static structure with a unique enzyme composition in each cisterna. Molecules are...
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Vesicular Tubular Clusters01:45

Vesicular Tubular Clusters

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After budding out from the ER membrane, some COPII vesicles lose their coat and fuse with one another to form larger vesicles and interconnected tubules called vesicular tubular clusters or VTCs. These clusters constitute a compartment at the ER-Golgi interface known as ERGIC (Endoplasmic Reticulum Golgi Intermediate Compartment). The ERGIC is a mobile membrane-bound cargo transport system that sorts proteins secreted from ER and delivers them to the Golgi.
With the help of motor proteins such...
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The Endoplasmic Reticulum01:43

The Endoplasmic Reticulum

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The endoplasmic reticulum or ER makes up for more than half of the membranes in a cell and accounts for 10% of total cell volume. It is also the primary protein and lipid synthesis factory for most cell organelles, such as the Golgi apparatus, lysosomes, secretory vesicles, and the plasma membrane. Despite being the most extensive and functionally complex subcellular organelle, ER was the last to be discovered. After years of deliberation, Keith Porter and George Palade in the year 1954,...
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Quantitative Localization of a Golgi Protein by Imaging Its Center of Fluorescence Mass
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The Golgi: Keeping It Unapologetically Basic.

Nathan P Ward1, Gina M DeNicola1

  • 1Department of Cancer Physiology, Moffitt Cancer Center, Tampa, Florida. gina.denicola@moffitt.org nathan.ward@moffitt.org.

Cancer Discovery
|June 3, 2020
PubMed
Summary
This summary is machine-generated.

Tumor cells create an acidic environment to fuel growth. Researchers found that NHE7 protein acidifies the trans-Golgi network, which is crucial for maintaining cancer cell pH and promoting tumor progression.

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Area of Science:

  • Oncology
  • Cell Biology
  • Biochemistry

Background:

  • Tumor cells exhibit altered pH gradients compared to normal cells.
  • This reverse pH gradient provides advantages for tumor survival and proliferation.

Purpose of the Study:

  • To investigate the role of NHE7 in regulating intracellular pH within tumor cells.
  • To determine the impact of NHE7-mediated pH changes on pancreatic ductal adenocarcinoma growth.

Main Methods:

  • Utilized molecular biology techniques to study NHE7 function.
  • Investigated the effect of NHE7 on the trans-Golgi network's pH.
  • Assessed the impact on cytosolic pH and tumor cell proliferation.

Main Results:

  • NHE7 was identified as a key mediator of trans-Golgi network acidification.
  • Acidification of the trans-Golgi network by NHE7 is essential for maintaining cytosolic pH.
  • This process is critical for sustaining pancreatic ductal adenocarcinoma growth.

Conclusions:

  • NHE7 plays a vital role in regulating tumor cell pH homeostasis.
  • Targeting NHE7 could represent a novel therapeutic strategy for pancreatic cancer.