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A pH-triggered G-triplex switch with K+ tolerance.

Heng Gao1, Xiong Zheng, Tong Yang

  • 1Key Laboratory of the Ministry of Education for Advanced Catalysis Materials, Institute of Physical Chemistry, College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua 321004, Zhejiang, China. yshao@zjnu.cn.

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A novel pH-triggered G-triplex (G3) switch utilizes potassium ions and a planar ligand for reversible iminium-alkanolamine conversion, controlling G3 structure formation and dissolution.

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Chemical Biology

Background:

  • G-quadruplex structures (G4) are G-rich nucleic acid secondary structures with diverse biological roles.
  • G-triplexes (G3) are less common but structurally distinct G-rich motifs.
  • Controlling G3 formation and dissolution is crucial for understanding their function and for developing novel molecular tools.

Purpose of the Study:

  • To demonstrate a pH-triggered switch for G-triplex (G3) formation and dissolution.
  • To utilize a planar ligand to initiate reversible G3 structuring and destructuring.
  • To investigate the role of potassium ions (K+) in G3 switch operation.

Main Methods:

  • Design and synthesis of a planar ligand capable of interacting with G-rich sequences.
  • Spectroscopic techniques (e.g., UV-Vis, fluorescence) to monitor G3 formation and conformational changes.
  • Electrochemical methods to assess the reversible iminium-alkanolamine conversion.

Main Results:

  • Demonstration of a pH-dependent G3 switch operating in the presence of K+.
  • The planar ligand successfully initiated reversible iminium-alkanolamine conversion.
  • This conversion acted as the trigger for G3 structuring and subsequent destructuring.

Conclusions:

  • A novel pH-triggered G3 switch mechanism has been successfully demonstrated.
  • The developed system offers a controllable method for G3 manipulation using a simple chemical trigger.
  • This work provides a foundation for developing G3-based molecular devices and sensors.