Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Conserved Binding Sites01:49

Conserved Binding Sites

5.0K
Many proteins’ biological role depends on their interactions with their ligands, small molecules that bind to specific locations on the protein known as ligand-binding sites. Ligand-binding sites are often conserved among homologous proteins as these sites are critical for protein function.
Binding sites are often located in large pockets, and if their location on a protein’s surface is unknown, it can be predicted using various approaches. The energetic method computationally...
5.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Structure-Controlled Molecular Recognition and Charge Transport in Metallized DNA Nanosheets.

Journal of the American Chemical Society·2026
Same author

DNA Framework Nucleator-Enabled Intelligent Hydrogel Interfaces on Living Cells.

Small (Weinheim an der Bergstrasse, Germany)·2026
Same author

Depolarization block paradoxically drives surges of neurotransmitter release during seizure activity.

Epilepsia·2026
Same author

Semiconductor Superlattice with Remarkable Raman Enhancement for Ultrafast Culture-Free Sensing of Multiple Pathogens.

Journal of the American Chemical Society·2026
Same author

Reply to the Correspondence on "Carbon-Dot-Based Dual-Emission Nanohybrid Produces a Ratiometric Fluorescent Sensor for in Vivo Imaging of Cellular Copper Ions".

Angewandte Chemie (International ed. in English)·2026
Same author

Machine learning-directed massively parallel programmable nucleic acid amplification.

Science advances·2026

Related Experiment Video

Updated: Dec 20, 2025

Author Spotlight: Developing Synthetic Cells from Programmable Amphiphilic DNA Nanostructures
08:02

Author Spotlight: Developing Synthetic Cells from Programmable Amphiphilic DNA Nanostructures

Published on: May 31, 2024

1.3K

Programming Biomimetically Confined Aptamers with DNA Frameworks.

Xiuhai Mao1, Mengmeng Liu2, Lei Yan1

  • 1Institute of Molecular Medicine, Shanghai Key Laboratory for Nucleic Acid Chemistry and Nanomedicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, China.

ACS Nano
|June 3, 2020
PubMed
Summary
This summary is machine-generated.

Framework nucleic acid (FNA) nanostructures enhance aptamer stability and binding affinity through molecular confinement. This strategy improves aptamer folding kinetics and catalytic activity, showing promise for biosensing applications.

Keywords:
DNA frameworkaptamerbiomimicrymolecular confinementthermodynamic stability

More Related Videos

Folding and Characterization of a Bio-responsive Robot from DNA Origami
07:59

Folding and Characterization of a Bio-responsive Robot from DNA Origami

Published on: December 3, 2015

15.0K
Designing a Bio-responsive Robot from DNA Origami
13:32

Designing a Bio-responsive Robot from DNA Origami

Published on: July 8, 2013

22.7K

Related Experiment Videos

Last Updated: Dec 20, 2025

Author Spotlight: Developing Synthetic Cells from Programmable Amphiphilic DNA Nanostructures
08:02

Author Spotlight: Developing Synthetic Cells from Programmable Amphiphilic DNA Nanostructures

Published on: May 31, 2024

1.3K
Folding and Characterization of a Bio-responsive Robot from DNA Origami
07:59

Folding and Characterization of a Bio-responsive Robot from DNA Origami

Published on: December 3, 2015

15.0K
Designing a Bio-responsive Robot from DNA Origami
13:32

Designing a Bio-responsive Robot from DNA Origami

Published on: July 8, 2013

22.7K

Area of Science:

  • Biochemistry
  • Nanotechnology
  • Molecular Biology

Background:

  • Protein active sites are confined within peptide scaffolds, creating microenvironments.
  • Tuning thermodynamic stability in biomimetic confinement faces challenges due to stoichiometry and reconfigurability.

Purpose of the Study:

  • To develop a framework nucleic acid (FNA)-based strategy for increasing aptamer thermodynamic stability.
  • To investigate the impact of molecular-scale confinement on aptamer folding kinetics and binding affinity.

Main Methods:

  • Utilized framework nucleic acid (FNA) structures to confine aptamers.
  • Employed single-molecule Förster resonance energy transfer (smFRET) to study folding kinetics.
  • Performed Monte Carlo simulations to analyze aptamer conformations.

Main Results:

  • Molecular confinement via FNAs significantly increased aptamer thermodynamic stability.
  • Facilitated folding kinetics and restricted unfavorable conformations were observed.
  • DNA framework-confined aptamers exhibited a ~3-fold improvement in binding affinity.
  • Enhanced catalytic activity was demonstrated for a hemin-binding aptamer.

Conclusions:

  • FNA-based molecular confinement is an effective strategy to enhance aptamer stability and function.
  • This approach offers potential for designing protein-mimicking DNA nanostructures for biosensing and biomedical applications.