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Updated: Dec 20, 2025

Genome Editing in Mammalian Cell Lines using CRISPR-Cas
Published on: April 11, 2019
CRISPR-TAPE: protein-centric CRISPR guide design for targeted proteome engineering
Daniel Paolo Anderson1, Henry James Benns1,2, Edward William Tate2
1Department of Life Sciences, Imperial College London, London, UK.
Abstract:
Rational molecular engineering of proteins with CRISPR-based approaches is challenged by the gene-centric nature of gRNA design tools. To address this, we have developed CRISPR-TAPE, a protein-centric gRNA design algorithm that allows users to target specific residues, or amino acid types within proteins. gRNA outputs can be customized to support maximal efficacy of homology-directed repair for engineering purposes, removing time-consuming post hoc curation, simplifying gRNA outputs and reducing CPU times.
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