Enhanced P450 fatty acid decarboxylase catalysis by glucose oxidase coupling and co-assembly for biofuel generation

Cytochrome P450 OleT is a fatty acid decarboxylase that uses hydrogen peroxide (H₂O₂) to catalyze the production of terminal alkenes, which are industrially important chemicals with biofuel and synthetic applications. Despite its requirement for large turnover levels, high concentrations of H₂O₂ may cause heme group degradation, diminishing enzymatic activity and limiting broad application for synthesis. Here, we report an artificial enzyme cascade composed of glucose oxidase (GOx) and OleT_SA from Staphylococcus aureus for efficient terminal alkene production. By adjusting the ratio of GOx to OleT_SA, the GOx-based tandem catalysis shows significantly improved product yield compared to the H₂O₂ injection method. Moreover, the co-assembly of the GOx/OleT_SA enzymes with a polymer, forming polymer-dual enzymes nanoparticles, displays improved activity compared to the free enzyme. This dual strategy provides a simple and efficient system to transform a naturally abundant feedstock to industrially important chemicals.