Jove
Visualize
Contact Us

Related Concept Videos

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

19.7K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
19.7K
Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

8.6K
Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
8.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Polarization-engineered aberration-resilient light sheet microscopy.

bioRxiv : the preprint server for biology·2026
Same author

Depth-enhanced molecular imaging with two-photon oblique plane microscopy.

ArXiv·2025
Same author

Neural Basis of Number Sense in Larval Zebrafish.

bioRxiv : the preprint server for biology·2024
Same author

A single-shot hyperspectral phasor camera for fast, multi-color fluorescence microscopy.

Cell reports methods·2023
Same author

Extended depth-of-field light-sheet microscopy improves imaging of large volumes at high numerical aperture.

Applied physics letters·2022
Same author

Publisher Correction: Author Correction: High-contrast, synchronous volumetric imaging with selective volume illumination microscopy.

Communications biology·2022
Same journal

A compact low-power magnetic particle imaging scanner based on a permanent-magnet field-free-line generator with high gradient.

The Review of scientific instruments·2026
Same journal

Achieving ultrahigh resolution with high efficiency: Optical design of the two-dimensional Resonant Inelastic X-ray Scattering (2D-RIXS) spectrometer at NanoTerasu beamline 02U.

The Review of scientific instruments·2026
Same journal

Automated laboratory x-ray diffractometer and fluorescence spectrometer for high-throughput materials characterization.

The Review of scientific instruments·2026
Same journal

Nonlinear Bayesian Doppler tomography for simultaneous reconstruction of flow and temperature.

The Review of scientific instruments·2026
Same journal

A Reflectance-based multimodal wearable photoplethysmography (PPG) sensor.

The Review of scientific instruments·2026
Same journal

Temporal analysis of products-Raman (TAP-Raman): An integrated setup for operando spectroscopy and transient kinetic analysis.

The Review of scientific instruments·2026
See all related articles
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Dec 20, 2025

Conducting Multiple Imaging Modes with One Fluorescence Microscope
08:32

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Published on: October 28, 2018

10.1K

A versatile, multi-laser twin-microscope system for light-sheet imaging.

Kevin Keomanee-Dizon1, Scott E Fraser2, Thai V Truong2

  • 1Translational Imaging Center, Dornsife College of Letters, Arts and Sciences, and Viterbi School of Engineering, University of Southern California, Los Angeles, California 90089, USA.

The Review of Scientific Instruments
|June 4, 2020
PubMed
Summary
This summary is machine-generated.

This study introduces a novel twin light-sheet microscope system to double imaging throughput and reduce costs for long-term biological dynamics studies. The versatile instrument enables flexible imaging parameters for diverse specimens, enhancing research efficiency.

More Related Videos

Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope
08:53

Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope

Published on: August 15, 2014

10.0K
Single Molecule Fluorescence Microscopy on Planar Supported Bilayers
20:00

Single Molecule Fluorescence Microscopy on Planar Supported Bilayers

Published on: October 31, 2015

14.3K

Related Experiment Videos

Last Updated: Dec 20, 2025

Conducting Multiple Imaging Modes with One Fluorescence Microscope
08:32

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Published on: October 28, 2018

10.1K
Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope
08:53

Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope

Published on: August 15, 2014

10.0K
Single Molecule Fluorescence Microscopy on Planar Supported Bilayers
20:00

Single Molecule Fluorescence Microscopy on Planar Supported Bilayers

Published on: October 31, 2015

14.3K

Area of Science:

  • Biophysics
  • Microscopy
  • Optical Imaging

Background:

  • Light-sheet microscopy enables fast, low-phototoxicity imaging for extended biological studies.
  • Current limitations include reduced specimen throughput for long-duration experiments.

Purpose of the Study:

  • To develop a versatile, high-throughput light-sheet imaging instrument.
  • To increase specimen imaging capacity and reduce costs for extended live-cell imaging.

Main Methods:

  • Designed a twin light-sheet microscope system sharing laser sources.
  • Incorporated flexible imaging parameters: one-photon/two-photon modes, multi-light-sheet illumination, variable resolution, multicolor capabilities, and adaptable detection geometry.
  • Provided detailed design for instrument builders.

Main Results:

  • Demonstrated increased throughput by utilizing two independently controlled microscopes.
  • Showcased versatility through imaging of zebrafish embryo heart, tumor organoids, and larval zebrafish brains.
  • Confirmed reduced phototoxicity and enhanced imaging speed for dynamic biological processes.

Conclusions:

  • The twin light-sheet microscope significantly enhances imaging throughput and cost-efficiency for long-term biological studies.
  • The instrument's flexibility supports diverse applications, from developmental biology to cancer research.
  • This design facilitates advanced live imaging of complex biological systems.