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Interaction Between CCL18 and GPR30 Differs from the Interaction Between Estradiol and GPR30.

Roland Schmidt-Wolf1, Gernot Zissel2

  • 1Department of Anesthesiology and Intensive Care Medicine, University Hospital Bonn, Bonn, Germany roland.schmidt-wolf@ukbonn.de.

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|June 4, 2020
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C-C motif chemokine ligand 18 (CCL18) activates the G protein-coupled receptor GPR30, but its signaling pathway differs from estradiol. This finding is crucial for understanding tumor progression and therapeutic outcomes.

Keywords:
Ccl18 proteincancerepithelial-mesenchymal transition (EMT)gpr30metastasis

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Area of Science:

  • Oncology
  • Molecular Biology
  • Endocrinology

Background:

  • C-C motif chemokine ligand 18 (CCL18) is overexpressed in tumors, promoting invasion and metastasis.
  • The G protein-coupled receptor GPR30, activated by both CCL18 and estradiol, is implicated in tumor progression.
  • Estradiol activation of GPR30 triggers pro-heparin-binding EGF-like growth factor and MAPK/ERK pathway transactivation.

Purpose of the Study:

  • To investigate whether CCL18 and estradiol utilize the same signaling pathway via GPR30.
  • To compare the downstream effects of CCL18 and estradiol on GPR30-mediated signaling in cancer cells.

Main Methods:

  • Utilized lung (A549) and breast (MCF-7, MDA-MB-231) cancer cell lines with varying GPR30 expression.
  • Stimulated cells with CCL18 in different media and analyzed gene and protein expression using qPCR, In-Cell Western®, western blot, and ELISA.

Main Results:

  • CCL18 and estradiol showed similarities in activating GPR30-mediated signaling, involving matrix metalloproteinases (MMPs) and ERK activation.
  • CCL18's effect on MMP7 expression varied with concentration, estrogen levels, and GPR30 inhibition, yielding unexpected results.
  • CCL18 induced differential ERK activation in A549 and MCF-7 cells, depending on co-stimulations, suggesting distinct pathway modulation.

Conclusions:

  • The G protein-coupled receptor GPR30 is integral to CCL18 signaling pathways.
  • CCL18 and estradiol, despite activating GPR30, may not elicit identical downstream signaling cascades.