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Knockdown of Myoferlin Suppresses Migration and Invasion in Clear-Cell Renal-Cell Carcinoma

Alexander Cox1, Chenming Zhao2, Yuri Tolkach3

  • 1Department of Urology, University Hospital Bonn, Bonn, Germany Alexander.Cox@ukbonn.de.

Anticancer Research
|June 4, 2020
PubMed
Abstract

Insights

Myoferlin (MYOF) is elevated in clear-cell renal-cell carcinoma (ccRCC), indicating its potential as a diagnostic biomarker for tumor progression and a therapeutic target.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biomarker Discovery

Background:

  • Myoferlin (MYOF) is an oncogenic protein implicated in various human cancers.
  • Its role in clear-cell renal-cell carcinoma (ccRCC) requires comprehensive investigation.

Purpose of the Study:

  • To investigate MYOF expression and function in ccRCC.
  • To evaluate MYOF as a diagnostic biomarker and therapeutic target for ccRCC.

Main Methods:

  • Assessed MYOF mRNA and protein expression using quantitative PCR and immunohistochemistry.
  • Performed siRNA-mediated MYOF knockdown in ACHN cells.
  • Conducted proliferation, migration, and invasion assays post-knockdown.

Main Results:

  • MYOF mRNA and protein were significantly upregulated in ccRCC, correlating with advanced tumor stage, higher grade, and positive lymph node/surgical margin status.
  • MYOF knockdown reduced migration and invasion in ACHN cells.
  • Knockdown led to upregulation of angiogenesis-associated genes TIE2, ANG2, and CAV1.

Conclusions:

  • MYOF is a potential diagnostic biomarker for ccRCC tumor progression.
  • MYOF represents a promising therapeutic target for ccRCC treatment.
Keywords:
Biomarkerimmunohistochemistryknockdownmyoferlinrenal cell carcinoma

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