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[Inhibitory effect of wogonin on human colorectal cancer cell SW480 based on network pharmacology]
Yan-Ping Li1, Yi-Ting Gong1, Jia-Xin Wang2
1School of Chinese Materia Medica, Beijing University of Chinese Medicine Beijing 100029, China.
Abstract:
Wogonin is a main effective component of Scutellaria baicalensis, with a significant anti-cancer activity. Recently, extensive studies focused on anti-cancer pharmacological effects of wogonin, but there were still a few studies on its molecular mechanism. Therefore, the molecular targets of its anti-cancer activity were still unclear. In this study, network pharmacology was applied to investigate the potential targets and molecular pathway of wogonin in inhibiting the growth of colorectal cancer. It indicated that Wnt/β-catenin was a key pathway of wogonin on colorectal cancer. Then, pharmacology and molecular mechanism studies were performed according to network pharmacological results. Pharmacological results revealed that wogonin inhibited significantly the proliferation of SW480(P<0.001), with a concentration-dependent regularity in the range of 12.5-50 μmol·L~(-1). Additionally, wogonin could induce G_1 phase blocking of SW480 cells. Western blot was used to investigate the effect of wogonin on four characteristic proteins of Wnt/β-catenin pathway. CTNNB1(β-catenin), BIRC5(survivin) and GSK3 B were down-regulated significantly, while the expression level of BAX was up-regulated(P<0.05). In conclusion, wogonin could inhibit the proliferation of SW480 cells through Wnt/β-catenin pathway. The feature protein CTNNB1(β-catenin), BIRC5(survivin), GSK3 B and BAX were identified as the potential targets. This study illuminated the anti-cancer molecular mechanism and drug targets of wogonin, which provided a theoretical basis for anti-colon cancer drug discovery and clinical application.
Insights
Wogonin, a compound from Scutellaria baicalensis, inhibits colorectal cancer cell growth by affecting the Wnt/β-catenin pathway. This study identifies key protein targets, offering insights for anti-colon cancer drug development.
Area of Science:
- Pharmacology
- Molecular Biology
- Network Pharmacology
Background:
- Wogonin, a key component of Scutellaria baicalensis, exhibits significant anti-cancer properties.
- The molecular mechanisms underlying wogonin's anti-cancer effects, particularly in colorectal cancer, remain largely unelucidated.
- Identifying molecular targets is crucial for developing wogonin as an anti-colon cancer therapeutic.
Purpose of the Study:
- To investigate the potential molecular targets and pathways of wogonin in inhibiting colorectal cancer growth using network pharmacology.
- To validate the anti-proliferative effects and molecular mechanism of wogonin in SW480 colorectal cancer cells.
- To identify specific protein targets involved in wogonin's anti-cancer activity.
Main Methods:
- Network pharmacology was employed to predict potential targets and pathways.
- Pharmacological assays were conducted to assess wogonin's effect on SW480 cell proliferation.
- Western blot analysis was used to examine the expression levels of key proteins in the Wnt/β-catenin pathway.
Main Results:
- Network pharmacology identified the Wnt/β-catenin pathway as a key mechanism for wogonin's anti-cancer activity.
- Wogonin significantly inhibited SW480 cell proliferation in a concentration-dependent manner and induced G1 phase arrest.
- Wogonin down-regulated CTNNB1 (β-catenin), BIRC5 (survivin), and GSK3B, while up-regulating BAX expression.
Conclusions:
- Wogonin effectively inhibits colorectal cancer cell proliferation by modulating the Wnt/β-catenin signaling pathway.
- Key molecular targets including CTNNB1, BIRC5, GSK3B, and BAX were identified.
- This research provides a theoretical foundation for the clinical application of wogonin in anti-colon cancer drug discovery.
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