Intraspecific variation of self-incompatibility in the distylous plant Primula merrilliana

Heteromorphic self-incompatibility can prevent self- and intramorph fertilization while favouring intermorph mating and the maintenance of morph-ratio stability in heterostylous populations. However, variation in the expression of self-incompatibility intraspecies has seldom been assessed. Through hand pollinations and microsatellite markers, the variation in the expression of self-incompatibility and genetic diversity were studied in distylous plant Primula merrilliana. We discovered that the strength of self-incompatibility varied extensively among individuals and populations, from pronounced to weaker self-incompatibility in distylous populations, all the way to strong self-compatibility in homostylous populations. Each distylous population included self-incompatible (SI), partly self-compatible (PSC) and self-compatible (SC) individuals, with the index of self-compatibility (ISC) ranging from 0.07 to 0.68 across populations. Self-compatible populations (ISC > 0.25) were not genetically clustered but were more closely related to populations with high SI and SC individuals were mixed with SI individuals within populations. The ISC and the proportions of SC and PSC individuals were higher in peripheral than in central populations, but no decrease of genetic diversity and no deviations of floral morph ratio from isoplethy were detected in peripheral populations. Additionally, the expression of self-incompatibility was stronger in long-styled flowers than in short-styled flowers. The variation in the strength of self-incompatibility documented in P. merrilliana cautions against the estimation of ISC from a few individuals or populations in distylous species and provides a more complex and nuanced understanding of the role of self-incompatibility in heterostyly.