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Cellular architecture response to aspect ratio tunable nanoarrays
Jing Dai1, Jinkang Gong, Na Kong
1School of Physical Science and Technology, ShanghaiTech University, 393 Middle Huaxia Road, Pudong, Shanghai 201210, China. yaoyuan@shanghaitech.edu.cn kongna@shanghaitech.edu.cn.
Nanoscale
|June 4, 2020
Summary
High aspect ratio nanoarrays significantly alter cell behavior, impacting attachment, viability, and motility. This research offers new insights into cell-surface interactions on nanostructured biointerfaces.
Area of Science:
- Biomaterials Science
- Cell Biology
- Nanotechnology
Background:
- Nanoarrays are valuable platforms for studying cell behavior and biological functions.
- Cell architecture responds to the biointerface presented by nanostructures.
Purpose of the Study:
- To develop tunable nanoarrays for investigating cell responses to nanostructure geometry.
- To understand how varying aspect ratios of nanostructures influence cell physiology.
Main Methods:
- Fabrication of tunable nanoarrays (nanoneedles, nanopillars, nanoclusters) using metal-assisted chemical etching.
- Controlled aspect ratio adjustments of nanostructures.
Main Results:
- High aspect ratio nanostructures (>10) significantly altered cell attachment, architecture, viability, proliferation, and motility.
- Cells on high aspect ratio nanostructures showed reorganized actin and vimentin filaments, with reduced focal adhesion.
Conclusions:
- Nanoarrays with high aspect ratios profoundly influence cell physiological behaviors.
- This study expands nanostructure diversity for nano-bio interface investigations.
- Findings provide insights into surface-dependent cell architecture and factors affecting cell physiology.
