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[Anticancer drug screening test for human osteosarcoma transplanted into nude mice]
1Department of Orthopaedic Surgery, Kyorin University School of Medicine, Tokyo, Japan.
Abstract:
Osteosarcoma tissue was transplanted into the subcutis of nude mice, and an anticancer drug was injected into the abdomen of the mice. The effects of Cis-platinum, SF-1739HP, Melphalan, Peleomycin and Aclacinomycin were tested. Only Cis-platinum had previously been used in the treatment of osteosarcoma. As the conventional method of evaluation, tumor weight change was recorded along the time course. As a new evaluation method, toluidine-blue was added into the tumor cell suspension prepared from the tumor tissue in the back of nude mice. By the intensity of staining, the tumor cells in suspension were classified into 3 categories; strongly-positive, weakly-positive and negative. Results of evaluation by the staining method were similar to those by measurement of tumor weight. Cis-platinum proved to be most effective, followed in decreasing order by SF-1739HP, Melphalan, Pepleomycin and Aclacinomycin. In conclusion, the staining method is simple and useful for screening the anti-cancer effects of drugs.
Insights
A novel toluidine-blue staining method effectively screens anticancer drug efficacy in osteosarcoma models. Cis-platinum demonstrated the highest effectiveness among tested drugs, validating the new method.
Area of Science:
- Oncology
- Pharmacology
- Biotechnology
Context:
- Osteosarcoma is a challenging bone cancer requiring effective therapeutic strategies.
- Preclinical models are crucial for evaluating novel anticancer agents.
- Traditional methods for assessing drug efficacy can be time-consuming.
Purpose:
- To evaluate the efficacy of five anticancer drugs against osteosarcoma xenografts in nude mice.
- To introduce and validate a novel toluidine-blue staining method for assessing drug-induced tumor cell viability.
- To compare the efficacy of Cis-platinum, SF-1739HP, Melphalan, Peleomycin, and Aclacinomycin.
Summary:
- Osteosarcoma tissue was xenografted into nude mice, and various anticancer drugs were administered.
- Tumor growth was monitored, and a new method involving toluidine-blue staining of tumor cell suspensions was developed.
- The staining method classified tumor cells into strongly-positive, weakly-positive, and negative categories, correlating well with traditional tumor weight measurements.
- Cis-platinum exhibited the most significant anticancer effect, followed by SF-1739HP, Melphalan, Peleomycin, and Aclacinomycin.
Impact:
- The toluidine-blue staining method provides a simple and efficient approach for screening anticancer drug effectiveness.
- This method can accelerate the discovery and development of new osteosarcoma treatments.
- Findings confirm Cis-platinum's potent activity and rank other agents, aiding future therapeutic decisions.