Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...

Sanger Sequencing

Sanger Sequencing

DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...

Next-generation Sequencing

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Multiple Genetic Shifts at the Crossroads of the Eastern Steppe and Loess Plateau Over 4,000 Years.

Nature communications·2026

Same author

Multidisciplinary analyses and ancient DNA reveal social inequality and mobility in the Central Plains during the Eastern Zhou period in China.

Nature human behaviour·2025

Same author

AATF alleviates cerebral ischemia-reperfusion injury by suppressing endoplasmic reticulum stress-induced ferroptosis through upregulating the PI3K/Akt pathway.

Neurological research·2025

Same author

Azacitidine monotherapy versus combination regimens as post-HSCT maintenance therapy in high-risk myeloid malignancies: a retrospective cohort study.

International journal of hematology·2025

Same author

Follow-up Optical Coherence Tomography Evaluation of Endothelialization in Drug-eluting Stents for Symptomatic Vertebral Artery Stenosis: a Report of Nine Cases.

Clinical neuroradiology·2025

Same author

Angiogenesis-driven hybrid hydrogel with pH/ROS-activated anti-infection and enhanced cellular metabolism for efficient MRSA-impaired wound repair.

Acta biomaterialia·2025

Search research articles

Related Experiment Video

Updated: Dec 19, 2025

Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations

Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations

Published on: August 4, 2016

High-coverage SARS-CoV-2 genome sequences acquired by target capture sequencing.

Shaoqing Wen^1,2, Chang Sun², Huanying Zheng³

¹Guangzhou Regenerative Medicine and Health Guangdong Laboratory, Guangzhou, China.

Journal of Medical Virology

|June 4, 2020

Summary

A new universal probe set effectively enriches SARS-CoV-2 sequences for comprehensive genome detection. This tool enhances viral detection capacity, especially in low-concentration clinical samples.

Keywords:

SARS coronavirus gene expression genetic networks genetic variability mutation

More Related Videos

In Vitro Selection of Aptamers to Differentiate Infectious from Non-Infectious Viruses

In Vitro Selection of Aptamers to Differentiate Infectious from Non-Infectious Viruses

Published on: September 7, 2022

Detecting Somatic Genetic Alterations in Tumor Specimens by Exon Capture and Massively Parallel Sequencing

Detecting Somatic Genetic Alterations in Tumor Specimens by Exon Capture and Massively Parallel Sequencing

Published on: October 18, 2013

Related Experiment Videos

Last Updated: Dec 19, 2025

Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations

Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations

Published on: August 4, 2016

In Vitro Selection of Aptamers to Differentiate Infectious from Non-Infectious Viruses

In Vitro Selection of Aptamers to Differentiate Infectious from Non-Infectious Viruses

Published on: September 7, 2022

Detecting Somatic Genetic Alterations in Tumor Specimens by Exon Capture and Massively Parallel Sequencing

Detecting Somatic Genetic Alterations in Tumor Specimens by Exon Capture and Massively Parallel Sequencing

Published on: October 18, 2013

Area of Science:

Virology
Genomics
Molecular Biology

Background:

Accurate detection and sequencing of SARS-CoV-2 are crucial for understanding viral evolution and controlling pandemics.
Existing methods may face challenges with low viral loads or require prior knowledge of viral subtypes.
Developing universal tools for efficient SARS-CoV-2 enrichment is essential for broad-spectrum detection.

Purpose of the Study:

To design and validate a universal SARS-CoV-2 enrichment probe set for enhanced sequence-based virus detection.
To enable simultaneous comprehensive genome sequencing of SARS-CoV-2.
To assess the probe set's effectiveness across various sample types and viral concentrations.

Main Methods:

Design of a universal probe set (502 x 120 nt single-stranded DNA biotin-labeled probes) based on all available SARS-CoV-2 sequences.
Application of the probe set for enrichment in cell culture, COVID-19 positive nasopharyngeal swabs (Ct 32-38), and negative throat swabs.
Sequencing of enriched samples to obtain high-quality viral genomes.

Main Results:

Demonstrated marked enrichment of SARS-CoV-2 sequences in clinical samples, including those with low viral loads (high Ct values).
Successfully obtained high-quality SARS-CoV-2 genome sequences, enabling further analysis.
Validated the universal nature of the probe set, independent of viral type or subtype.

Conclusions:

The developed universal SARS-CoV-2 enrichment probe system effectively captures and enriches viral sequences.
This system significantly enhances the capacity for sequence-based virus detection and comprehensive genome sequencing.
The probe set is particularly valuable for analyzing clinical samples with low viral particle concentrations.