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Base Excision Repair

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Related Experiment Video

Updated: Dec 19, 2025

Functional Assessment of BRCA1 variants using CRISPR-Mediated Base Editors

Functional Assessment of BRCA1 variants using CRISPR-Mediated Base Editors

Published on: February 28, 2021

Cas12a Base Editors Induce Efficient and Specific Editing with Low DNA Damage Response.

Xiao Wang¹, Chengfeng Ding¹, Wenxia Yu¹

¹School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China; Shanghai Institute of Biochemistry and Cell Biology, CAS Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences, Shanghai 200031, China; University of Chinese Academy of Sciences, Beijing 100049, China.

|June 4, 2020

Summary

This summary is machine-generated.

New base editors (BEs) using Cas12a minimize DNA damage and activate fewer DNA damage response proteins. The BEACON system achieves efficient C-to-T editing with high specificity, even in vivo.

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Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

Area of Science:

Molecular Biology
Gene Editing
Biotechnology

Background:

Base editors (BEs) offer potential for correcting disease-causing point mutations.
Cas9 nickase (nCas9)-derived BEs can cause unwanted DNA double-strand breaks and DNA damage response (DDR).

Purpose of the Study:

To develop a novel base editor system that minimizes DNA damage and DDR.
To enhance deamination efficiency and editing specificity compared to existing systems.

Main Methods:

Conjugating catalytically dead Cas12a (dCas12a) with engineered human apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3A (APOBEC3A) to create the BEACON system.
Evaluating BEACON's DNA break induction, DDR activation (H2AX, ATM, ATR, p53), C-to-T editing efficiency, and RNA off-target mutations in mammalian cells.
Assessing in vivo base editing in mouse embryos and F0 mice.

Main Results:

dCas12a-conjugated BEs induced minimal DNA breaks and DDR.
The BEACON system achieved efficient C-to-T editing comparable to AncBE4max with low DDR and minimal RNA off-target mutations.
BEACON successfully induced in vivo base editing in mouse embryos, with targeted C-to-T conversions observed in F0 mice.

Conclusions:

The BEACON system represents a promising advancement in base editing technology, offering enhanced efficiency and specificity while minimizing unwanted DNA damage and DDR.
BEACON's ability to perform in vivo base editing in mammalian embryos opens new avenues for therapeutic applications in genetic diseases.