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Estimation of basophil-bound IgE by quantitative immunofluorescence microscopy
International Archives of Allergy and Applied Immunology
|January 1, 1977
Summary
This study quantifies immunoglobulin E (IgE) on human basophils using immunofluorescence and microfluorometry. Atopic patients
Area of Science:
- Immunology
- Cell Biology
Background:
- Basophils play a crucial role in allergic reactions.
- Quantifying surface-bound immunoglobulin E (IgE) on basophils is essential for understanding allergic responses.
Purpose of the Study:
- To develop and validate a method for measuring IgE load on human basophils.
- To assess the correlation between IgE levels and allergic status.
Main Methods:
- Human leukocyte suspensions were treated with anti-IgE immunofluorescence.
- Basophil fluorescence intensity was measured using microfluorometry.
- IgE was eluted and quantified at pH 2.5.
Main Results:
- A reproducible technique for measuring IgE on basophils was established.
- Basophils from atopic patients exhibited significantly higher IgE levels.
- A strong correlation was observed between microfluorometry readings and eluted IgE quantity.
- The number of IgE molecules per basophil ranged from 15,000 to 500,000.
Conclusions:
- Microfluorometry provides a reliable measure of IgE load on basophils.
- This method can differentiate IgE levels between healthy donors and atopic individuals.
- The findings contribute to a better understanding of basophil involvement in atopy.