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Related Experiment Video

Updated: Dec 19, 2025

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Temperature-programmable and enzymatically solidifiable gelatin-based bioinks enable facile extrusion bioprinting.

Huimin He1,2, Duo Li1,2,3, Zifeng Lin1

  • 1Research Center for Human Tissue and Organs Degeneration, Institute of Biomedicine and Biotechnology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, People's Republic of China.

Biofabrication
|June 4, 2020
PubMed
Summary

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Chemically modified gelatin bioinks with ureido-pyrimidinone (UPy) and tyramine (Tyr) offer enhanced printability and fidelity for tissue engineering. These bioinks support high cell viability and function in complex 3D constructs.

Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Polymer Chemistry

Background:

  • Developing advanced bioinks for extrusion bioprinting is crucial for creating functional tissue constructs.
  • Gelatin is biocompatible but lacks the viscosity and stable gelation required for high-fidelity bioprinting.
  • Existing gelatin-based bioinks often compromise printability or cell viability.

Purpose of the Study:

  • To engineer a modified gelatin bioink with improved printability, fidelity, and cell viability for extrusion bioprinting.
  • To develop a bioink with temperature-programmable viscosity and enzyme-controlled solidification.
  • To demonstrate the potential of the modified gelatin for fabricating complex, cell-laden tissue constructs.

Main Methods:

  • Sequential chemical modification of gelatin with ureido-pyrimidinone (UPy) and tyramine (Tyr) moieties.

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  • Utilizing temperature-dependent viscosity and enzyme-triggered gelation for controlled printing and solidification.
  • Fabricating various 2D and 3D cell-laden constructs, including scaffolds and tissue-like structures, using extrusion bioprinting.

    • Main Results:

    • The modified Gel-UPy-Tyr bioink exhibited enhanced printability and superior fidelity in construct fabrication.
    • Cell-laden constructs demonstrated high cell viability (>90% at 24h) and maintained cell proliferation and function over one week.
    • Successful creation of diverse constructs, including 2D patterns, 3D scaffolds, and multi-cellular tissue mimics.

    Conclusions:

    • Chemically modified gelatin (Gel-UPy-Tyr) provides a promising platform for advanced extrusion bioprinting.
    • The developed bioink overcomes the limitations of native gelatin, enabling high-fidelity fabrication of viable cell-laden constructs.
    • This approach supports cell growth and function, paving the way for engineered tissues and regenerative medicine applications.