Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cost-Effectiveness of a Practical Approach to Prevent Respiratory Infection Outbreaks in Long-Term Care Settings: The CARES Strategy.

American journal of infection control·2026
Same author

Increased abundance of Limosilactobacillus reuteri in the gut of selectively bred high-tameness mice and its association with behavioural changes.

DNA research : an international journal for rapid publication of reports on genes and genomes·2026
Same author

Distinct Concentration-dependent dsDNA-binding Modes of a Dinoflagellate Cold Shock Domain Protein Provide Insight into Mechanisms of Nuclear Regulation.

Plant & cell physiology·2026
Same author

The SPARK complex forms the molecular basis of vertebrate fertilization.

bioRxiv : the preprint server for biology·2026
Same author

Rat DCST1 and DCST2 are essential for sperm-egg fusion†.

Biology of reproduction·2026
Same author

Reticulate evolution, introgression, and recent diversification in Epimedium sect. Macroceras.

Molecular phylogenetics and evolution·2026

Related Experiment Video

Updated: Dec 19, 2025

Author Spotlight: Exploring Microglial Interactions with Stress-Response Circuitry Using the Limited Bedding and Nesting Model
04:20

Author Spotlight: Exploring Microglial Interactions with Stress-Response Circuitry Using the Limited Bedding and Nesting Model

Published on: July 12, 2024

2.3K

Reduction in BDNF from Inefficient Precursor Conversion Influences Nest Building and Promotes Depressive-Like

Masami Kojima1,2,3, Hikari Otabi4, Haruko Kumanogoh1,2

  • 1Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 1-8-31 Midorioka, Ikeda, Osaka 563-8577, Japan.

International Journal of Molecular Sciences
|June 5, 2020
PubMed
Summary

Impaired cleavage of brain-derived neurotrophic factor (BDNF) precursor (proBDNF) to mature BDNF (mBDNF) in mice caused depressive-like behaviors and deficits in daily activities. This suggests reduced mBDNF contributes to stress-induced phenotypes.

Keywords:
BDNFSNPcleavagedepressionnest buildingsocial isolation

More Related Videos

Nest Building Behavior as an Early Indicator of Behavioral Deficits in Mice
06:11

Nest Building Behavior as an Early Indicator of Behavioral Deficits in Mice

Published on: October 19, 2019

20.9K
Applying Stereotactic Injection Technique to Study Genetic Effects on Animal Behaviors
07:54

Applying Stereotactic Injection Technique to Study Genetic Effects on Animal Behaviors

Published on: May 10, 2015

14.6K

Related Experiment Videos

Last Updated: Dec 19, 2025

Author Spotlight: Exploring Microglial Interactions with Stress-Response Circuitry Using the Limited Bedding and Nesting Model
04:20

Author Spotlight: Exploring Microglial Interactions with Stress-Response Circuitry Using the Limited Bedding and Nesting Model

Published on: July 12, 2024

2.3K
Nest Building Behavior as an Early Indicator of Behavioral Deficits in Mice
06:11

Nest Building Behavior as an Early Indicator of Behavioral Deficits in Mice

Published on: October 19, 2019

20.9K
Applying Stereotactic Injection Technique to Study Genetic Effects on Animal Behaviors
07:54

Applying Stereotactic Injection Technique to Study Genetic Effects on Animal Behaviors

Published on: May 10, 2015

14.6K

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Genetics

Background:

  • Brain-derived neurotrophic factor (BDNF) is crucial for neuronal plasticity and function.
  • Dysregulation of BDNF signaling is implicated in various neurological and psychiatric disorders.
  • The proteolytic processing of proBDNF to mBDNF is a critical regulatory step.

Purpose of the Study:

  • To investigate the functional consequences of impaired proBDNF to mBDNF conversion.
  • To model human single nucleotide polymorphisms affecting proBDNF cleavage in mice.
  • To determine the role of reduced mBDNF in stress-induced behaviors.

Main Methods:

  • Generation of a knock-in mouse line with human proBDNF polymorphisms (R125M, R127L).
  • Biochemical analysis of proBDNF/mBDNF ratios in hippocampal lysates.
  • Behavioral assessments including tail suspension test and nest-building assays.
  • Evaluation of weight gain under social isolation conditions.

Main Results:

  • Mutant mice exhibited a dose-dependent decrease in the mBDNF/proBDNF ratio.
  • Heterozygous mice showed a 57% reduction in mBDNF levels.
  • Mutant mice displayed depressive-like behaviors (tail suspension test) and weight gain during social isolation.
  • Socially isolated heterozygous mice showed deficits in nest-building behavior.

Conclusions:

  • Impaired proBDNF cleavage significantly reduces mBDNF levels.
  • Reduced mBDNF production contributes to stress-induced depressive-like phenotypes.
  • Deficits in mBDNF signaling disrupt normal daily activities and behaviors.