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Convergent genes shape budding yeast pericentromeres.

Flora Paldi1, Bonnie Alver1, Daniel Robertson1

  • 1The Wellcome Centre for Cell Biology, Institute of Cell Biology, School of Biological Sciences, University of Edinburgh, Edinburgh, UK.

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|June 5, 2020
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Summary
This summary is machine-generated.

Budding yeast pericentromeres organize genome structure and function. Convergent genes and cohesin positioning shape these regions, ensuring proper chromosome segregation during mitosis.

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Area of Science:

  • Genomics
  • Molecular Biology
  • Cell Biology

Background:

  • The three-dimensional genome architecture is crucial for its maintenance, expression, and transmission.
  • The cohesin protein complex organizes the genome by linking distant loci and is enriched in pericentromeres.

Purpose of the Study:

  • To elucidate the 3D structure of pericentromeres in budding yeast (Saccharomyces cerevisiae).
  • To establish the relationship between pericentromere genome organization and cellular function, particularly chromosome segregation.

Main Methods:

  • Investigated the 3D structure of pericentromeres in Saccharomyces cerevisiae.
  • Analyzed the role of convergent genes and cohesin in defining pericentromere structure and function.
  • Examined the impact of gene reorientation on pericentromere organization and chromosome biorientation.

Main Results:

  • Convergent genes at pericentromere borders, along with core centromeres, define structure and position cohesin.
  • Pericentromeres adopt a looped conformation with border genes at the base; microtubule attachment extends these loops.
  • Reorienting border genes impairs cohesin positioning, enlarges pericentromeres, and disrupts chromosome biorientation.

Conclusions:

  • The linear arrangement of genes and targeted cohesin loading shape pericentromeres for competent chromosome segregation.
  • Microtubule attachment restructures pericentromere architecture.
  • A direct, causal link exists between 3D genome organization and cellular function in pericentromeres.