Robust Magnetized Oil Palm Leaves Ash Nanosilica Composite as Lipase Support: Immobilization Protocol and Efficacy...

Strategies to immobilize the individual enzymes are crucial for enhancing catalytic applicability and require a controlled immobilization process. Herein, protocol for immobilizing Candida rugosa lipase (CRL) onto modified magnetic silica derived from oil palm leaves ash (OPLA) was optimized for the effects of concentration of CRL, immobilization time, and temperature, monitored by titrimetric and spectrometric methods. XRD and TGA-DTG spectrometric observations indicated that OPLA-silica was well coated over magnetite (SiO₂-MNPs) and CRLs were uniformly bound by covalent bonds to SiO₂-MNPs (CRL/Gl-A-SiO₂-MNPs). The optimized immobilization protocol showed that in the preparation of CRL/Gl-A-SiO₂-MNPs, CRL with 68.3 mg/g protein loading and 74.6 U/g specific activity was achieved using 5 mg/mL of CRL, with an immobilization time of 12 h at 25 °C. The present work also demonstrated that acid-pretreated OPLA is a potential source of renewable silica, envisioning its applicability for practical use in enzymatic catalysis on solid support.