[Study on rapid quality analysis method of Shengxuebao Mixture]

Area of Science:

• Analytical Chemistry
• Spectroscopy
• Herbal Medicine Analysis

Background:

• Quality control of traditional Chinese medicine like Shengxuebao Mixture is crucial.
• Accurate quantification of multiple active ingredients is challenging with conventional methods.

Purpose of the Study:

• To develop and validate a rapid, simultaneous analysis method for eight active ingredients in Shengxuebao Mixture.
• To establish a quality evaluation system for Shengxuebao Mixture using spectroscopic techniques.

Main Methods:

• Development of a rapid analysis method utilizing ultraviolet-visual (UV-Vis) and near-infrared (NIR) spectroscopy.
• Simultaneous detection of eight active ingredients: albiflorin, paeoniflorin, 2,3,5,4'-tetra-hydroxy-stilbene-2-O-β-D-glucopyranoside, specnuezhenide, ecliptasaponin D, emodin, calycosin-7-glucoside, and astragaloside Ⅳ.
• Multivariate data analysis, specifically Partial Least Squares (PLS) regression, was employed to build calibration models.

Main Results:

• UV-Vis and NIR models demonstrated excellent calibration performance (R² > 0.9).
• Prediction ability of the models was satisfactory, with R² values exceeding 0.83 for UV-Vis and 0.79 for NIR.
• The method accurately quantified eight active ingredients compared to HPLC-UV-MS reference analysis.

Conclusions:

• The established UV-Vis and NIR spectroscopic method provides an accurate, robust, and fast approach for Shengxuebao Mixture quality evaluation.
• This technique offers a viable alternative for routine quality control of complex herbal formulations.
• The method facilitates efficient monitoring of active ingredient content, ensuring product consistency and efficacy.