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Circular RNA_LARP4 inhibits cell migration and invasion of prostate cancer by targeting FOXO3A

X-D Weng1, T Yan, C-L Liu

  • 1Department of Urology Surgery, Yinzhou People's Hospital, Yinzhou, China. qdzxyymnwk@163.com.

European Review for Medical and Pharmacological Sciences
|June 5, 2020
PubMed
Abstract

Insights

Circular RNA_LARP4 acts as a tumor suppressor in prostate cancer (PCa). Low expression of circular RNA_LARP4 correlates with poor prognosis, and its overexpression inhibits PCa cell migration and invasion by upregulating FOXO3A.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cancer Research

Background:

  • Circular RNAs (circRNAs) are increasingly recognized for their roles in various cancers.
  • Circular RNA_LARP4 has been identified as a tumor suppressor in gastric cancer.
  • The specific function of circular RNA_LARP4 in prostate cancer (PCa) development is not well understood.

Purpose of the Study:

  • To investigate the role and mechanism of circular RNA_LARP4 in prostate cancer.
  • To determine if circular RNA_LARP4 functions as a tumor suppressor or oncogene in PCa.

Main Methods:

  • Quantitative Polymerase Chain Reaction (RT-qPCR) to measure circular RNA_LARP4 expression in PCa tissues and cell lines.
  • Functional assays including wound healing and transwell assays to assess cell migration and invasion.
  • Western blot analysis to evaluate the protein expression of FOXO3A.
  • Lentivirus-mediated overexpression of circular RNA_LARP4 in PCa cells.

Main Results:

  • Circular RNA_LARP4 expression was significantly downregulated in PCa tissues and cell lines compared to adjacent normal tissues.
  • Low circular RNA_LARP4 expression was associated with poorer prognosis in PCa patients.
  • Overexpression of circular RNA_LARP4 inhibited the migration and invasion capabilities of PCa cells.
  • Circular RNA_LARP4 overexpression led to increased expression of FOXO3A.

Conclusions:

  • Circular RNA_LARP4 functions as a tumor suppressor in prostate cancer.
  • The tumor-suppressive effect of circular RNA_LARP4 in PCa is mediated through the upregulation of FOXO3A, thereby inhibiting cell migration and invasion.

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