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LncNORAD interference inhibits tumor growth and lung cancer cell proliferation, invasion and migration by

Y Wu1, Q-W Shen, Y-X Niu

  • 1Department of Thoracic Surgery, Shanghai Key Laboratory of Clinical Geriatric Medicine, HuaDong Hospital, FuDan University, Shanghai, China. Shenxy2019@yeat.net.

European Review for Medical and Pharmacological Sciences
|June 5, 2020
PubMed
Abstract

Insights

Downregulating long non-coding RNA NORAD inhibits non-small cell lung cancer (NSCLC) progression. This study shows NORAD interference suppresses tumor growth and metastasis by affecting CXCR4 and the RhoA/ROCK pathway.

Area of Science:

  • Oncology
  • Molecular Biology
  • Gene Regulation

Background:

  • Non-small cell lung cancer (NSCLC) presents a significant clinical challenge due to poor survival rates.
  • Identifying novel diagnostic and therapeutic targets is crucial for improving NSCLC outcomes.
  • Long non-coding RNAs (lncRNAs) are increasingly recognized for their roles in cancer development.

Purpose of the Study:

  • To investigate the role of the lncRNA NORAD in the proliferation, invasion, migration, and tumor growth of NSCLC.
  • To elucidate the underlying molecular mechanisms involving CXCR4, CXCL12, and the RhoA/ROCK pathway.

Main Methods:

  • Quantitative real-time PCR (RT-PCR) to measure NORAD mRNA levels.
  • Cell viability assays (CCK-8), wound healing, and Transwell assays to assess proliferation and cell migration/invasion.
  • Immunohistochemistry and Western blot to analyze protein expression (CXCR4, CXCL12, RhoA/ROCK pathway components).
  • In vivo NSCLC mouse models to evaluate NORAD's effect on tumor growth.

Main Results:

  • NORAD expression was significantly elevated in NSCLC tissues and cells.
  • Interference with NORAD markedly inhibited tumor growth and suppressed NSCLC cell proliferation, migration, and invasion.
  • NORAD downregulation led to decreased expression of CXCR4 and CXCL12.
  • The RhoA/ROCK signaling pathway was found to be activated in NSCLC.

Conclusions:

  • Downregulation of lncRNA NORAD can impede NSCLC progression.
  • NORAD influences NSCLC by regulating CXCR4 and the RhoA/ROCK signaling pathway.
  • Targeting NORAD may offer a potential therapeutic strategy for NSCLC.

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