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MiR-424 suppressed viability and invasion by targeting to the DCLK1 in neuroblastoma
1Department of Pediatrics, The First Affiliated Hospital of Shandong First Medical University, Jinan, China. changkuuhgds83623@163.com.
Objective:
Neuroblastoma is the most frequent tumor of sympathetic nervous system in infants. MiRNAs acted as oncogenes or tumor suppressors in the process of tumor development. We aim at exploring the functions of miRNA in neuroblastoma.
Patients And Methods:
Cell viability and invasion were evaluated by Cell Counting Kit-8 (CCK-8) and transwell assays. Western blot was utilized to assess the protein expression associated with epithelial-mesenchymal transition (EMT) markers. Quantitative Real Time-Polymerase Chain Reaction (qRT-PCR) was performed to calculate the mRNA levels of miRNA and gene.
Results:
MiR-424 was downregulated while doublecortin like kinase 1 (DCLK1) was upregulated in neuroblastoma tissues and cells compared to adjacent non-tumor and normal spongiocyte cells. MiR-424 suppressed cell viability, invasion, and EMT by targeting DCLK1. MiR-424 regulated the expression of DCLK1 by directly binding to the 3'-untranslated region (UTR) of DCLK1 mRNA in SK-N-SH and Be2C cells. DCLK1 reversed partial functions of miR-424 in neuroblastoma.
Conclusions:
MiR-424 suppressed cell viability, invasion, and EMT by directly targeting the 3'-UTR of DCLK1 mRNA. The newly identified miR-424/DCLK1 axis provides novel insights into the pathogenesis of neuroblastoma.
Insights
MicroRNA-424 (miR-424) suppresses neuroblastoma growth and spread by targeting doublecortin like kinase 1 (DCLK1). This newly found miR-424/DCLK1 pathway offers new understanding of neuroblastoma development.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Neuroblastoma is a common infant cancer originating from the sympathetic nervous system.
- MicroRNAs (miRNAs) play crucial roles as oncogenes or tumor suppressors in cancer development.
Purpose of the Study:
- To investigate the function of specific miRNAs in neuroblastoma pathogenesis.
- To explore the relationship between miR-424 and its potential targets in neuroblastoma cells.
Main Methods:
- Cell Counting Kit-8 (CCK-8) and transwell assays were used to assess cell viability and invasion.
- Western blot analysis was performed to evaluate epithelial-mesenchymal transition (EMT) markers.
- Quantitative Real Time-Polymerase Chain Reaction (qRT-PCR) was used to measure miRNA and gene mRNA levels.
Main Results:
- MiR-424 was found to be downregulated, while doublecortin like kinase 1 (DCLK1) was upregulated in neuroblastoma tissues and cells.
- MiR-424 demonstrated tumor-suppressive effects by inhibiting cell viability, invasion, and EMT.
- MiR-424 directly targeted the 3'-untranslated region (UTR) of DCLK1 mRNA, regulating its expression.
Conclusions:
- MiR-424 suppresses neuroblastoma cell viability, invasion, and EMT by directly targeting DCLK1 mRNA.
- The identified miR-424/DCLK1 axis provides novel insights into the molecular mechanisms underlying neuroblastoma development.
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