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Effect of ciRS-7 expression on clear cell renal cell carcinoma progression

Yan-Hui Zhao1, Zhi Wang2, Na Zhang1

  • 1Departments of Urology, Qingdao Central Hospital, Qingdao, Shandong 266042, China.

Chinese Medical Journal
|June 5, 2020
PubMed
Abstract

Insights

Circular RNA ciRS-7 is overexpressed in clear cell renal cell carcinoma (ccRCC), promoting tumor progression and metastasis. Targeting ciRS-7 may offer a new therapeutic strategy for ccRCC patients.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Circular RNA ciRS-7 (circRNA ciRS-7) is implicated in various cancers.
  • Its role in clear cell renal cell carcinoma (ccRCC) progression is not well understood.
  • This study investigates circRNA ciRS-7 expression and function in ccRCC.

Purpose of the Study:

  • To determine the expression levels of circRNA ciRS-7 in ccRCC tissues.
  • To elucidate the role of circRNA ciRS-7 in ccRCC cell proliferation and invasion.
  • To explore the underlying molecular mechanisms, including the EGFR/Akt signaling pathway.

Main Methods:

  • Quantitative reverse transcription polymerase chain reaction (qRT-PCR) for circRNA ciRS-7 expression analysis.
  • Cell counting kit-8 and invasion assays to assess cell proliferation and invasion.
  • Western blotting to investigate signaling pathway activation.
  • Statistical analysis including Chi-square test and Kaplan-Meier survival analysis.

Main Results:

  • circRNA ciRS-7 was significantly overexpressed in ccRCC tissues compared to normal tissues.
  • Higher circRNA ciRS-7 levels correlated with larger tumor diameter, advanced clinical stage, and distant metastasis.
  • Knockdown of circRNA ciRS-7 suppressed ccRCC cell proliferation and invasion.
  • circRNA ciRS-7 inhibition reduced levels of phosphorylated epidermal growth factor receptor (p-EGFR) and phosphorylated Akt (p-Akt).

Conclusions:

  • Upregulated circRNA ciRS-7 promotes ccRCC cell proliferation and invasion.
  • This promotion may involve the epidermal growth factor receptor (EGFR)/Akt signaling pathway.
  • circRNA ciRS-7 represents a potential therapeutic target for ccRCC.

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