Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

EPS and iPS Cells in Disease Research01:21

EPS and iPS Cells in Disease Research

3.2K
Embryonic and induced pluripotent stem cells are excellent models for disease research because of their ability to self-renew and differentiate into most cell types. Somatic cells from a patient are isolated and reprogrammed into induced pluripotent stem cells or iPSCs. These iPSCs are later differentiated into the desired cell type, which mirrors the diseased cell of the patient. In this way, disease models have been created for investigating diseases such as Down syndrome, type I diabetes,...
3.2K
Induced Pluripotent Stem Cells01:13

Induced Pluripotent Stem Cells

27.1K
Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore...
27.1K
Induced Pluripotent Stem Cells01:06

Induced Pluripotent Stem Cells

5.2K
Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic...
5.2K
Somatic to iPS Cell Reprogramming01:29

Somatic to iPS Cell Reprogramming

2.5K
Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012...
2.5K
Maintenance of the ES Cell State01:14

Maintenance of the ES Cell State

2.6K
The cells of the blastocyst inner cell mass only remain pluripotent for a short time. This state of pluripotency and self-renewal can be maintained in embryonic stem (ES) cell culture by adding specific chemicals or growth factors to ensure the cells can continue dividing and later differentiate into different cell types. In some cases, the cells are grown on a feeder layer of differentiated cells, which provides the growth factors and extracellular matrix components necessary for stem cell...
2.6K
Stem Cell Culture01:17

Stem Cell Culture

5.9K
Stem cell research aims to find ways to use stem cells to regenerate and repair cellular damage. Over time, most adult cells undergo the wear and tear of aging and lose their ability to divide and repair themselves. Stem cells do not display a particular morphology or function. Adult stem cells, which exist as a small subset of cells in most tissues, keep dividing and can differentiate into a number of specialized cells generally formed by that tissue. These cells enable the body to renew and...
5.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

RNA-seq at different stages of human pancreatic β cell differentiation reveals proliferation dynamics and SMAD9 in directing β cell fate.

Cell death & disease·2026
Same author

Models of hyperglycaemia in diabetes mellitus and its complications.

Nature reviews. Endocrinology·2026
Same author

Stem cell therapies for diabetes.

Nature medicine·2025
Same author

ZHX3 interacts with CEBPB to repress hepatic gluconeogenic gene expression and uric acid secretion.

PNAS nexus·2025
Same author

HNF4A and HNF1A exhibit tissue specific target gene regulation in pancreatic beta cells and hepatocytes.

Nature communications·2024
Same author

Computationally defined and in vitro validated putative genomic safe harbour loci for transgene expression in human cells.

eLife·2024
Same journal

Brent A. Reynolds, pioneer of adult neural stem cell biology.

Stem cells (Dayton, Ohio)·2026
Same journal

CircVapa promotes the abnormal differentiation of small intestinal epithelial stem cells in diabetic state.

Stem cells (Dayton, Ohio)·2026
Same journal

Transforming Growth Factor beta-2 (TGFβ2) Drives Trabecular Meshwork Progenitor Cell Differentiation Through SMAD2/3 Signalling.

Stem cells (Dayton, Ohio)·2026
Same journal

Circular RNA circEGFR overexpression attenuates chemosensitivity and enhances cancer stemness via targeting IGF2BP2/SOX2 in breast cancer cells.

Stem cells (Dayton, Ohio)·2026
Same journal

Regeneration of the mammalian brain: a relic of evolution?

Stem cells (Dayton, Ohio)·2026
Same journal

Mitochondrial transfer technologies with molecular insights into clinical applications.

Stem cells (Dayton, Ohio)·2026
See all related articles

Related Experiment Video

Updated: Dec 19, 2025

A Novel Culture Model for Human Pluripotent Stem Cell Propagation on Gelatin in Placenta-conditioned Media
07:33

A Novel Culture Model for Human Pluripotent Stem Cell Propagation on Gelatin in Placenta-conditioned Media

Published on: August 3, 2015

8.2K

Replicates in stem cell models-How complicated!

Jun-Wei Chan1,2, Adrian K K Teo1,3

  • 1Stem Cells and Diabetes Laboratory, Institute of Molecular and Cell Biology (IMCB), A*STAR, Singapore, Singapore.

Stem Cells (Dayton, Ohio)
|June 5, 2020
PubMed
Summary
This summary is machine-generated.

Human pluripotent stem cell (hPSC) research involves complex steps, from patient recruitment and reprogramming to cell differentiation and analysis. These studies aim to understand disease mechanisms by comparing healthy and patient-derived stem cells.

More Related Videos

Selecting and Isolating Colonies of Human Induced Pluripotent Stem Cells Reprogrammed from Adult Fibroblasts
13:23

Selecting and Isolating Colonies of Human Induced Pluripotent Stem Cells Reprogrammed from Adult Fibroblasts

Published on: February 20, 2012

20.3K
Patterning the Geometry of Human Embryonic Stem Cell Colonies on Compliant Substrates to Control Tissue-Level Mechanics
10:04

Patterning the Geometry of Human Embryonic Stem Cell Colonies on Compliant Substrates to Control Tissue-Level Mechanics

Published on: September 28, 2019

8.7K

Related Experiment Videos

Last Updated: Dec 19, 2025

A Novel Culture Model for Human Pluripotent Stem Cell Propagation on Gelatin in Placenta-conditioned Media
07:33

A Novel Culture Model for Human Pluripotent Stem Cell Propagation on Gelatin in Placenta-conditioned Media

Published on: August 3, 2015

8.2K
Selecting and Isolating Colonies of Human Induced Pluripotent Stem Cells Reprogrammed from Adult Fibroblasts
13:23

Selecting and Isolating Colonies of Human Induced Pluripotent Stem Cells Reprogrammed from Adult Fibroblasts

Published on: February 20, 2012

20.3K
Patterning the Geometry of Human Embryonic Stem Cell Colonies on Compliant Substrates to Control Tissue-Level Mechanics
10:04

Patterning the Geometry of Human Embryonic Stem Cell Colonies on Compliant Substrates to Control Tissue-Level Mechanics

Published on: September 28, 2019

8.7K

Area of Science:

  • Biomedical Research
  • Stem Cell Biology
  • Genetics

Background:

  • Human pluripotent stem cell (hPSC) research is crucial for disease modeling.
  • Patient-specific induced pluripotent stem cells (hiPSCs) offer a powerful tool for studying genetic diseases.
  • Current methodologies present significant complexities in hPSC-based studies.

Purpose of the Study:

  • To outline the current complexities in human pluripotent stem cell (hPSC)-based studies.
  • To detail the process from patient recruitment to phenotypic analysis.
  • To highlight challenges in disease modeling using hiPSCs.

Main Methods:

  • Recruitment of patients with disease-associated gene variants.
  • Somatic reprogramming to generate patient-specific hiPSCs.
  • Directed differentiation of hiPSCs to relevant cell types.
  • Qualitative/quantitative assays for phenotypic and gene expression analysis.

Main Results:

  • The process involves multiple intricate steps, each with potential challenges.
  • Comparison of healthy and diseased hiPSCs allows for disease mechanism investigation.
  • The study highlights the step-wise nature of hPSC-based disease modeling.

Conclusions:

  • hPSC-based studies are complex, requiring careful execution of each stage.
  • Understanding these complexities is vital for advancing disease research using stem cells.
  • Patient-derived hiPSCs are instrumental in dissecting disease-specific cellular phenotypes.