Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Microbial Fermentation

Microbial Fermentation

Fermentation is a crucial anaerobic metabolic process that enables microbes to derive energy from sugar without relying on oxygen or an electron transport chain. This process is fundamental to various biological and industrial applications and is classified based on the metabolic products generated.Role of Pyruvate in FermentationPyruvate and its derivatives serve as key electron acceptors in fermentative pathways. The oxidation of NADH to regenerate NAD+ is essential for the continuation of...

Biosynthesis in Bacteria

Biosynthesis in Bacteria

Biosynthesis in bacteria is a fundamental anabolic process that generates essential macromolecules, including proteins, nucleic acids, lipids, and polysaccharides. These macromolecules are critical for cellular growth, replication, and function. The process is tightly regulated and energetically linked to catabolic pathways to ensure optimal resource utilization.Biosynthetic pathways begin with precursor metabolites such as pyruvate, acetyl-CoA, and glucose-6-phosphate derived from glycolysis,...

Techniques for Isolation of Pure Cultures

Techniques for Isolation of Pure Cultures

Microorganisms are routinely cultured in the laboratory using various techniques to isolate, grow, and quantify them for further study. These methods rely on inoculating microorganisms into a suitable growth medium under aseptic conditions to prevent contamination. Depending on the objective, inoculation can involve direct transfer or the use of diluted bacterial suspensions as the inoculum.Streak-Plate Method for IsolationThe streak-plate method is a common technique for obtaining pure...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

A Novel De Novo Frameshift Variant of TBL1XR1 Leads to Neurodevelopmental Disorders and Treatment Experience.

International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience·2026

Same author

Systems-Level Metabolic Regulation Strategies of Xylose Metabolism in Saccharomyces cerevisiae.

Biotechnology journal·2026

Same author

Biomanufacturing of Therapeutically Relevant Chondroitin Sulfate C via Engineered Microbes.

ACS synthetic biology·2026

Same author

<i>LAMA2</i> variants associated with muscular dystrophy, brain structural abnormalities, and epilepsy: a genotype-phenotype study.

Frontiers in neurology·2026

Same author

Engineering the gut microbiome and its impact on human health.

Current opinion in biotechnology·2025

Same author

Novel KDM3B Variants in Two Chinese Patients With Global Developmental Delay and Autism.

International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience·2025

Search research articles

Related Experiment Video

Updated: Dec 19, 2025

Biosynthesis of a Flavonol from a Flavanone by Establishing a One-pot Bienzymatic Cascade

Biosynthesis of a Flavonol from a Flavanone by Establishing a One-pot Bienzymatic Cascade

Published on: August 14, 2019

Microbial Coculture for Flavonoid Synthesis.

Peng Xu¹, Monireh Marsafari¹, Jian Zha²

¹Department of Chemical, Biochemical and Environmental Engineering, University of Maryland Baltimore County, MD 21250, USA.

Trends in Biotechnology

|June 5, 2020

Summary

This summary is machine-generated.

Microbial cocultures enhance flavonoid biosynthesis, offering a cost-effective and diverse production method for these health-promoting plant compounds. This approach expands the capabilities beyond single microbial systems.

Keywords:

flavonoids host selection microbial coculture pathway efficiency population dynamics structural diversity

More Related Videos

Assembly and Quantification of Co-Cultures Combining Heterotrophic Yeast with Phototrophic Sugar-Secreting Cyanobacteria

Assembly and Quantification of Co-Cultures Combining Heterotrophic Yeast with Phototrophic Sugar-Secreting Cyanobacteria

Published on: December 27, 2024

Using Coculture to Detect Chemically Mediated Interspecies Interactions

Using Coculture to Detect Chemically Mediated Interspecies Interactions

Published on: October 31, 2013

Related Experiment Videos

Last Updated: Dec 19, 2025

Biosynthesis of a Flavonol from a Flavanone by Establishing a One-pot Bienzymatic Cascade

Biosynthesis of a Flavonol from a Flavanone by Establishing a One-pot Bienzymatic Cascade

Published on: August 14, 2019

Assembly and Quantification of Co-Cultures Combining Heterotrophic Yeast with Phototrophic Sugar-Secreting Cyanobacteria

Assembly and Quantification of Co-Cultures Combining Heterotrophic Yeast with Phototrophic Sugar-Secreting Cyanobacteria

Published on: December 27, 2024

Using Coculture to Detect Chemically Mediated Interspecies Interactions

Using Coculture to Detect Chemically Mediated Interspecies Interactions

Published on: October 31, 2013

Area of Science:

Biotechnology
Metabolic Engineering
Synthetic Biology

Background:

Flavonoids are plant-derived compounds with significant human health benefits.
The flavonoid biosynthetic pathway is complex and modular.
Current methods for flavonoid production face limitations in cost-efficiency and diversity.

Purpose of the Study:

To explore the potential of microbial cocultures for flavonoid biosynthesis.
To leverage the metabolic diversity of different microbial hosts.
To improve the cost-efficiency and structural diversity of flavonoid production.

Main Methods:

Utilizing the modularity of the flavonoid biosynthetic pathway.
Employing distinct microbial hosts in coculture systems.
Installing novel structural functionalities through metabolic engineering.

Main Results:

Microbial cocultures enable the combination of metabolic characteristics from different hosts.
This approach allows for the creation of flavonoid structures not achievable in monocultures.
Potential for improved cost-efficiency and expanded diversity in flavonoid biosynthesis.

Conclusions:

Microbial cocultures represent a promising strategy for advancing flavonoid biosynthesis.
This technology can overcome limitations of traditional single-host systems.
Future applications include cost-effective and diverse production of valuable flavonoids.