Antibodies to oxidized lipoproteins in human normal plasma, detection and analysis

In the article, presence of antibodies to protein components of oxidized human donor plasma was demonstrated. It was shown that normal plasma oxidized by CuCl₂, FeSO₄, or potassium ferricyanide acquired ability to bind with human IgG-sensitized polystyrene latex particles and colloidal gold nanoparticles. Ability of these antibodies to bind to oxidized components strongly depends on time of oxidation. Moreover, Sepharose 2B conjugated with human IgG has affinity to component of such plasma. Molecular weight analysis demonstrates that this component eluted as a single peak in size exclusion chromatography with molecular weight about 3 × 10⁶ Da.