Related Experiment Video
Updated: Dec 19, 2025
Author Spotlight: Advancements in DNA Nanosensors – Addressing Sensitivity and Selectivity Challenges in Molecular Detection
Published on: February 9, 2024
Zwitterionic polymer chain-assisted lysozyme imprinted core-shell carbon microspheres with enhanced recognition and
Liwei Qian1, Wenqian Liu2, Miaoxiu Yang2
1College of Bioresources Chemical and Materials Engineering, Shaanxi University of Science and Technology, Xi'an, 710021, China; School of Natural and Applied Science, Northwestern Polytechnical University, Xi'an, 710072, China.
Researchers developed a novel protein imprinting material using zwitterionic polymer chains for enhanced lysozyme recognition. This new material, CFC-PVSP@MIPs, shows superior selectivity and simplified preparation for protein imprinting technology applications.
Area of Science:
- Materials Science
- Biotechnology
- Analytical Chemistry
Background:
- Protein imprinting technology (PIT) faces challenges in creating materials with high protein recognition and selectivity.
- Developing efficient and selective imprinted materials is crucial for various applications, including diagnostics and purification.
Purpose of the Study:
- To prepare a core-shell carbon microsphere imprinted material (CFC-PVSP@MIPs) with enhanced recognition and selectivity for lysozyme.
- To investigate the role of a zwitterionic polymer chain (Poly (1-vinyl-3-sulfopropylimidazolium), PVSP) in improving imprinting performance.
Main Methods:
- Utilized template immobilization and surface imprinting technologies to create core-shell carbon microspheres.
- Incorporated carboxyl-functionalized carbon microspheres as a substrate and dopamine as a functional monomer and crosslinker.
- Introduced PVSP via non-covalent interaction to form the imprinted layer.
Main Results:
- The CFC-PVSP@MIPs demonstrated a high adsorption capacity (68.1 mg g⁻¹) and a fast adsorption equilibrium rate (120 min).
- The imprinted material exhibited superior recognition ability for lysozyme with an imprinting factor of 3.10, compared to PVSP-free materials (1.93).
- The zwitterionic groups in PVSP enhanced selectivity in competitive adsorption studies.
Conclusions:
- The proposed strategy offers a promising and convenient method for preparing protein imprinted materials with high recognition and selectivity.
- The developed CFC-PVSP@MIPs are conducive to the advancement and application of protein imprinting technology.
- The zwitterionic polymer chain significantly improves the performance of imprinted materials by reducing non-specific adsorption and enhancing recognition.
