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Updated: Dec 19, 2025

A Bright NIR-II Fluorescence Probe for Vascular and Tumor Imaging

A Bright NIR-II Fluorescence Probe for Vascular and Tumor Imaging

Published on: March 17, 2023

Depth-resolved NIR-II fluorescence mesoscope.

Jiuling Liao^1,2, Yixuan Yin^1,2, Jia Yu^1,2

¹Research Laboratory for Biomedical Optics and Molecular Imaging, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China.

Biomedical Optics Express

|June 6, 2020

Summary

This study introduces a new Near-Infrared II (NIR-II) fluorescence mesoscopy system for deep tissue imaging. The system achieves a large field of view, high resolution, and deep penetration, enabling advanced visualization of biological structures.

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Last Updated: Dec 19, 2025

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Fluorescence Imaging with One-nanometer Accuracy FIONA

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Published on: September 26, 2014

Area of Science:

Biomedical optics
Medical imaging technologies
Fluorescence microscopy

Background:

Near-Infrared II (NIR-II) fluorescence imaging offers deep tissue visualization due to reduced scattering and autofluorescence.
Existing NIR-II methods struggle to balance field of view, resolution, penetration depth, and optical sectioning.

Purpose of the Study:

To develop a novel NIR-II fluorescence mesoscopy system overcoming current limitations.
To enable high-performance deep tissue imaging with enhanced capabilities.

Main Methods:

Implementation of an f-θ scanning scheme for mesoscopic imaging.
Integration of confocal detection for optical sectioning and high resolution.
Utilizing NIR-II fluorescent dyes for imaging.

Main Results:

The system images samples up to 7.5×7.5 mm² with a lateral resolution of 6.3 µm.
Achieved depth-resolved imaging with zooming capabilities.
Successfully demonstrated in vivo cerebrovascular imaging in a mouse model of local ischemia.

Conclusions:

The developed NIR-II mesoscopy system provides a powerful tool for deep tissue imaging.
Offers new possibilities for studying cerebrovascular diseases and their mechanisms.