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Calcium Fluorescence Recordings from Neuroepithelial Stem Cells.
1Center for Basic Medical Research, International University of Health and Welfare, Ohtawara, Japan. my57@iuhw.ac.jp.
Methods in Molecular Biology (Clifton, N.J.)
|June 6, 2020
Summary
Neuroepithelial cells function as neural stem cells during embryonic development. This chapter details calcium (Ca2+) fluorescence recording techniques to study their physiological properties.
Area of Science:
- Developmental Biology
- Neuroscience
- Cell Biology
Background:
- Neuroepithelial cells serve as neural stem cells during embryonic development.
- These cells exhibit tight intercellular connections and basement membrane contact.
- Understanding their physiological properties is crucial for developmental neuroscience.
Purpose of the Study:
- To describe detailed techniques for calcium (Ca2+) fluorescence recording.
- To enable the study of physiological properties in neuroepithelial stem cells.
Main Methods:
- Calcium (Ca2+) fluorescence recording.
- Microscopy techniques for live cell imaging.
- Cell preparation for physiological assays.
Main Results:
- Successful application of Ca2+ fluorescence recording in neuroepithelial stem cells.
- Demonstration of techniques for monitoring cellular activity.
- Provides a foundation for further physiological studies.
Conclusions:
- Calcium (Ca2+) fluorescence recording is a viable method for studying neuroepithelial stem cells.
- The described techniques facilitate research into neural stem cell physiology.
- This approach aids in understanding early embryonic neural development.
