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MIR22HG regulates miR-486/PTEN axis in bladder cancer to promote cell proliferation

Qisheng Tang1, Xue Jiang2, Shanjin Ma1

  • 1Department of Urology, Tangdu Hospital, Air Force Medical University, Xi'an City, Shaanxi Province 710038, China.

Bioscience Reports
|June 6, 2020
PubMed

Insights

MIR22HG suppresses bladder cancer (BC) by regulating the miR-486/PTEN axis. Down-regulation of MIR22HG promotes BC cell proliferation, while its overexpression inhibits it.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • The tumor-suppressive function of MIR22HG is recognized across various cancers.
  • MIR22HG's role in bladder cancer (BC) pathogenesis requires further elucidation.

Purpose of the Study:

  • To investigate the role of MIR22HG in bladder cancer.
  • To explore the molecular mechanism of MIR22HG in regulating BC cell proliferation.

Main Methods:

  • Analysis of The Cancer Genome Atlas (TCGA) dataset for MIR22HG expression in BC.
  • Bioinformatics analysis and dual-luciferase reporter assays to confirm MIR22HG-miR-486 interaction.
  • Cell proliferation assays to assess the functional impact of MIR22HG, PTEN, and miR-486.

Main Results:

  • MIR22HG was found to be down-regulated in bladder cancer.
  • MIR22HG directly interacts with miR-486 but does not affect its expression.
  • MIR22HG overexpression up-regulates PTEN, inhibiting BC cell proliferation, an effect reversed by miR-486.

Conclusions:

  • MIR22HG acts as a tumor suppressor in bladder cancer.
  • MIR22HG regulates BC cell proliferation through the miR-486/PTEN axis.
Keywords:
MIR22HGPTENbladder cancermiR-486proliferation

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