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Related Experiment Video

Updated: Dec 19, 2025

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A novel nucleic acid amplification system based on nano-gap embedded active disk resonators.

Eun Yeong Lee1, Yeseul Kim2, Bonhan Koo1

  • 1Department of Convergence Medicine, Asan Medical Institute of Convergence Science and Technology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, 05505, Republic of Korea.

Sensors and Actuators. B, Chemical
|June 6, 2020
PubMed
Summary

A novel whispering gallery mode resonator amplifies nucleic acids directly on a bio-optical sensor. This method achieves single-copy detection in minutes, paving the way for simple, sensitive point-of-care molecular diagnostics.

Keywords:
Molecular diagnosisOptical sensorPoint-of-care testingSilicon-rich silicon nitride diskWhispering gallery mode

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Area of Science:

  • Biophotonics
  • Molecular Diagnostics
  • Nanotechnology

Background:

  • Existing nucleic acid tests often rely on hybridization, lacking amplification.
  • Direct amplification on optical sensors faces challenges like sensitivity, complexity, and high background signals.

Purpose of the Study:

  • To introduce a novel nucleic acid amplification method using a whispering gallery mode active resonator.
  • To explore its potential for molecular diagnostics with enhanced sensitivity and simplicity.

Main Methods:

  • Utilized a whispering gallery mode active resonator with implanted nanoclusters.
  • Operated the resonator without tapered fiber coupling for photoluminescence emission.
  • Leveraged nano-gap structures for strong light-matter interaction.

Main Results:

  • Achieved a strong photoluminescence signal with low background in aqueous environments.
  • Demonstrated an extremely low detection threshold, down to a single nucleic acid copy.
  • Realized detection within 10 minutes due to efficient light-matter interaction.

Conclusions:

  • The whispering gallery mode active resonator offers a new approach for direct nucleic acid amplification.
  • This technology enables high refractive index contrast, tight mode confinement, and simple alignment.
  • The system holds promise for developing low-cost, high-sensitivity, and simple point-of-care molecular diagnostic devices.