Chromatography of biogenic amines. I. Generally applicable separation and detection methods

Area of Science:

• Analytical Chemistry
• Biochemistry

Background:

• Biogenic amines are crucial in various biological processes.
• Accurate separation and detection of biogenic amines are essential for research.
• Existing chromatographic methods require comprehensive review.

Purpose of the Study:

• To review generally applicable separation and detection methods for biogenic amines.
• To compare the usefulness of different derivative-forming reactions for amine analysis.
• To provide a foundation for understanding advanced chromatographic techniques in Part II.

Main Methods:

• Review of paper chromatography, thin-layer chromatography, paper electrophoresis, thin-layer electrophoresis, and ion-exchange methods.
• Analysis of reagents for forming colored and fluorescent amine derivatives.
• Comparison of derivative-forming reactions based on available data.

Main Results:

• General separation and detection techniques for biogenic amines are described.
• Various reagents for derivatization are reviewed, with applications discussed.
• The relative utility of different derivative-forming reactions is compared, acknowledging data limitations.

Conclusions:

• Established chromatographic and electrophoretic techniques are fundamental for biogenic amine analysis.
• Derivatization plays a key role in enhancing amine detectability.
• Further research is needed to fully elucidate the optimal methods for specific amine determinations.