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Mast cell activation markers for in vitro study.

Muhammad Novrizal Abdi Sahid1,2, Takeshi Kiyoi3

  • 1Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Gadjah Mada , Yogyakarta, Indonesia.

Journal of Immunoassay & Immunochemistry
|June 6, 2020
PubMed
Summary

This review details mast cell (MC) activation indicators, including histamine and β-hexosaminidase. Understanding these components aids in predicting experimental outcomes for mast cell research.

Keywords:
Mast cell activationgranulehistaminein vitromediator release

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Area of Science:

  • Immunology
  • Cell Biology

Background:

  • Mast cells (MCs) are key players in allergic reactions.
  • MC activation by secretagogues leads to increased intracellular calcium, granule trafficking, and mediator release via exocytosis.
  • Three exocytosis types (piecemeal, kiss-and-run, compound) contribute to differential mediator release.

Purpose of the Study:

  • To review mast cell components used as activation indicators.
  • To explain the principles behind determining these components.
  • To guide the selection of appropriate indicators for specific research designs.

Main Methods:

  • Review of literature on mast cell activation markers.
  • Explanation of methodologies for measuring mediators like histamine and β-hexosaminidase.
  • Discussion of techniques for observing granule trafficking and eicosanoid release.

Main Results:

  • Classical markers include histamine and β-hexosaminidase.
  • Eicosanoids and granule trafficking provide further insights into MC activation status.
  • Different exocytosis mechanisms correlate with the release of specific mediators.

Conclusions:

  • Accurate measurement of mast cell mediators is crucial for assessing activation.
  • This review provides a framework for selecting appropriate indicators.
  • Informed selection of indicators enhances the predictability of experimental results in mast cell studies.