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Related Experiment Videos

Liposome-encapsulated hemoglobin: a synthetic red cell.

M C Farmer1, S A Johnson, R L Beissinger

  • 1Bio/Molecular Engineering Branch, Naval Research Laboratory, Washington, D.C. 20375-5000.

Advances in Experimental Medicine and Biology
|January 1, 1988
PubMed
Summary

Liposome-encapsulated hemoglobin (LEH) offers a universally transfusable blood replacement. This study details a scalable method for producing sterile LEH with properties suitable for clinical applications.

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Area of Science:

  • Biomedical Engineering
  • Materials Science
  • Hematology

Background:

  • Liposome-encapsulated hemoglobin (LEH) is a potential oxygen-carrying blood substitute.
  • Previous limitations in production scale and sterility hindered toxicity testing.
  • Development of a universally transfusable blood replacement is critical.

Purpose of the Study:

  • To develop a scalable and sterile method for producing LEH.
  • To characterize the biophysical properties of LEH for blood replacement applications.
  • To optimize LEH formulation for extended circulation persistence.

Main Methods:

  • Utilized a Microfluidizer™ for liposome production (0.2 micron).
  • Employed Pellicon tangential flow filtration for washing and sterile filtration.

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  • Co-encapsulated pyridoxal-5-phosphate to maintain oxygen-binding affinity.

    • Main Results:

    • Achieved production of liters of LEH with ≥16 g% hemoglobin in hours.
    • Developed a formulation with a circulation persistence time of 15-20 hours.
    • Demonstrated LEH biophysical properties mimic red blood cells.

    Conclusions:

    • The developed method overcomes previous production and sterility barriers.
    • LEH exhibits promising characteristics for use as a blood substitute.
    • Further research into lipid composition and circulation dynamics is warranted.